Atmospheric pressure matrix-assisted laser desorption/ionization (AP/MALDI) mass spectrometry (MS) allows for highly sensitive and specific detection and relative quantification of metabolites of interest, with the key benefit of minimal sample preparation requirements for complex biological samples such as bacteria. Violacein is a violet-colored pigment produced by the bacterium Chromobacterium violaceum. In this chapter, we detail a high-throughput, high-resolution (HR) MS-based, targeted metabolite profiling protocol for analyzing violacein and its intermediates directly from bacterial colonies. This technique applies to a wide range of metabolites regulated by operon control and is suitable for a molecular phenotyping platform.

Keywords: AP/MALDI MS; Adaptive laboratory evolution (ALE); Antibiotic resistance; Chromobacterium violaceum; Metabolomics; Microbial analysis; Pathway metabolites; Vio operon.

© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

大气压基质辅助激光解吸电离（AP/MALDI）质谱（MS）能够对感兴趣的代谢物进行高灵敏度和特异性检测以及相对定量，其关键优势在于对复杂生物样本（如细菌）的样品制备要求极低。紫黑精是一种由紫色杆菌（Chromobacterium violaceum）产生的紫色色素。在本章中，我们详细描述了一种基于高通量、高分辨率（HR）MS的目标代谢物分析方法，用于直接从细菌菌落中分析紫黑精及其中间体。该技术适用于受操纵子调控的多种代谢物，并且适合分子表型平台。

关键词： AP/MALDI MS；适应性实验室进化（ALE）；抗生素耐药性；紫色杆菌；代谢组学；微生物分析；途径代谢物；Vio操纵子。

© 2025. 作者保留独家授权给Springer Science+Business Media, LLC，Springer Nature的一部分的权利。