Background: Diabetic wounds are one of the most common complications of diabetes mellitus. Jingfang Granules (JFG), a combination of 11 herbs, has been clinically used for treating colds and the flu and for preventing various skin diseases.

Purpose: The present study was designed to evaluate the therapeutic effect of JFG on diabetic wounds and to elucidate the associated mechanisms.

Methods: JFG serum was prepared using Sprague-Dawley rats and the phytochemicals of JFG in the serum were identified using UHPLC-ESI-QE-Orbitrap-MS. A cell viability assay and cellular angiogenesis methods were performed to evaluate wound healing in vitro. Diabetic wounds were developed using streptozotocin-induced diabetic rats to investigate the efficacy of JFG on diabetic wounds in vivo. Network pharmacology analysis, molecular docking, and Western blot were performed to elucidate the potential mechanisms of JFG in diabetic wound healing.

Results: JFG serum attenuated H₂O₂-induced and high glucose-induced oxidative damage, significantly reduced lipopolysaccharide-induced upregulation of inflammatory cytokines, and promoted angiogenesis in vitro. In diabetic rats, JFG effectively promoted wound healing, reduced blood glucose and lipid levels, and alleviated oxidative stress and inflammation. A total of 56 phytochemicals were identified in the JFG serum. Six core targets (AKT1, EGFR, MAPK3, MAPK1, IL6, and TNF) and the PI3K-AKT and MAPK signaling pathways were identified by network pharmacology analysis, which were further validated by subsequent experimental methods.

Conclusion: JFG could accelerate diabetic wound healing by alleviating oxidative damage, suppressing inflammation, promoting angiogenesis, and regulating metabolic abnormalities, with involvement of the PI3K-AKT and MAPK signaling pathways.

Keywords: MAPK; PI3K-AKT; diabetic wounds; jingfang granules; network pharmacology.

© 2025 Wang et al.

背景：

糖尿病足溃疡是糖尿病最常见的并发症之一。荆防颗粒（JFG）是由11种草药组成的复方制剂，临床上用于治疗感冒和流感，并预防各种皮肤病。

目的：

本研究旨在评估荆防颗粒对糖尿病创面的治疗效果，并阐明其相关机制。

方法：

使用Sprague-Dawley大鼠制备荆防颗粒血清，并通过UHPLC-ESI-QE-Orbitrap-MS技术鉴定荆防颗粒在血清中的植物化学成分。进行细胞活力测定和细胞血管生成实验，评估体外创面愈合效果。利用链脲佐菌素诱导的糖尿病大鼠模型发展糖尿病伤口，以研究荆防颗粒对糖尿病伤口的效果。通过网络药理学分析、分子对接以及Western blot技术，阐明荆防颗粒在糖尿病创面愈合中的潜在机制。

结果：

荆防颗粒血清可以减轻H₂O₂-诱导和高血糖诱导的氧化损伤，显著降低脂多糖诱导的炎症细胞因子上调，并促进体外血管生成。在糖尿病大鼠中，荆防颗粒能够有效促进伤口愈合、降低血糖和血脂水平，减轻氧化应激及炎症反应。总共鉴定了56种植物化学成分存在于荆防颗粒血清中。通过网络药理学分析发现6个核心靶点（AKT1, EGFR, MAPK3, MAPK1, IL6 和 TNF）以及PI3K-AKT和MAPK信号通路，这些结果被后续实验方法进一步验证。

结论：

荆防颗粒通过减轻氧化损伤、抑制炎症反应、促进血管生成及调节代谢异常等方式加速糖尿病创面愈合，并且涉及到了PI3K-AKT和MAPK信号通路的参与。