There is a drive towards reducing animal experiments and developing robust biologically relevant in vitro models based on cell lines, including those derived from fish. At the time of writing, Cellosaurus, the knowledge base of current cell lines used in research, listed more than 900 fish cell lines in its database. One of the key challenges facing fish cell biology is the lack of fundamental technical information regarding the isolation, culture, and application of cell lines. Researchers often work in silos, encountering similar technical challenges, each spending significant time and resources overcoming the same issues for which solutions may not be readily accessible. Here, we share some of the key considerations for the isolation, culture, maintenance, and application of fish cell lines in toxicology, which we have encountered over our collective decades of experience.

Keywords: Cell line development; Fish cell culture; In vitro toxicology; Invitromatics; Primary culture.

© 2025. The Author(s).

目前有一种减少动物实验的趋势，即开发基于细胞系（包括鱼类来源的细胞系）的强大且具有生物相关性的体外模型。截至撰写本文时，Cellosaurus（一个当前研究中使用的细胞系的知识库），在其数据库中列出了超过900种鱼类细胞系。鱼类细胞生物学面临的一个主要挑战是缺乏有关细胞系分离、培养和应用的基本技术信息。研究人员往往各自为战，在遇到相似的技术难题时，每个人都要花费大量时间和资源来克服这些问题，而这些解决方案可能并不容易获取。在这里，我们分享了一些在毒理学中关于鱼类细胞系的分离、培养、维持和应用的关键考虑因素，这是基于我们在相关领域几十年的经验。

关键词： 细胞系开发；鱼类细胞培养；体外毒理学；Invitromatics；原代培养。

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