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American journal of reproductive immunology (New York, N.Y. : 1989). 2025 Apr;93(4):e70071. doi: 10.1111/aji.70071 Q32.52024

Effect of COVID-19 Infection During Pregnancy on the Plasma/Extracellular Vesicles Pro-Inflammatory Cytokine Profile

妊娠期感染新冠病毒对血浆/外泌体促炎症细胞因子谱的影响 翻译改进

C Heeralall  1, Usri H Ibrahim  2, M Jenneker  3, S Singh  4, L Lazarus  1, Irene Mackraj  2

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作者单位

  • 1 Discipline of Clinical Anatomy, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
  • 2 Discipline of Human Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
  • 3 Discipline of Obstetrics and Gynaecology, School of Clinical Medicine, University of KwaZulu-Natal, Durban, South Africa.
  • 4 Optics & Imaging Centre, Doris Duke Medical Research Institute, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.

    • DOI: 10.1111/aji.70071 PMID: 40198239

    摘要 中英对照阅读

    Purpose: The Coronavirus disease (COVID-19) has impacted pregnant women significantly, with increased mortality and morbidity. The implications of this virus are linked to extracellular vesicles (EVs) and maternal inflammation due to the cytokine storm. Hence, this study aims to investigate the impact of COVID-19 on the pro-inflammatory cytokine profile in both plasma and EVs of South African pregnant women.

    Methods: Plasma samples were obtained from pregnant women in the third trimester, from which EVs were extracted using the Invitrogen Total Exosome Isolation Kit. These plasma-derived EVs were characterised using transmission electron microscopy and nanoparticle tracking analysis (NTA).

    Results: COVID-19 infection in pregnancy did not significantly affect the average particle size and concentration of isolated EVs. The levels of IFN gamma, IL-6, MIP-1 alpha and TNF alpha were analysed in the plasma and circulating EVs through a multiplex assay. Compared to the control group, a significant increase in IL-6, IFN-γ, TNF-α and MIP-1α levels were observed in both plasma and EVs content of COVID-19 pregnancies.

    Conclusion: These findings suggest that COVID-19 infection impacts the pro-inflammatory cytokine profile in the plasma and EVs of South African pregnant women.

    Keywords: COVID‐19; cytokine storm; extracellular vesicles; inflammation; pregnancy.

    Keywords：covid-19 infection; pregnancy; plasma/extracellular vesicles

    目的： 新型冠状病毒（COVID-19）对孕妇产生了重大影响，导致了更高的死亡率和发病率。这种病毒的影响与细胞外囊泡（EVs）以及由于细胞因子风暴引发的母体炎症相关联。因此，本研究旨在调查 COVID-19 对南非孕妇血浆及 EV 中促炎性细胞因子谱的影响。

    方法： 从孕晚期的孕妇处获取了血浆样本，并使用 Invitrogen Total Exosome Isolation Kit 提取了其中的 EVs。利用透射电子显微镜和纳米颗粒跟踪分析（NTA）对这些血浆来源的 EVs 进行了表征。

    结果： 怀孕期间 COVID-19 感染并未显著影响分离出的 EV 的平均粒子大小和浓度。通过多重测定法分析了血浆及循环 EV 中 IFN gamma、IL-6、MIP-1 alpha 和 TNF alpha 的水平。与对照组相比，COVID-19 孕妇的血浆和 EV 内 IL-6、IFN-γ、TNF-α 和 MIP-1α 水平均有显著增加。

    结论： 这些发现表明，COVID-19 感染影响了南非孕妇血浆及 EV 中的促炎性细胞因子谱。

    关键词： COVID‐19；细胞因子风暴；细胞外囊泡；炎症；妊娠。

    关键词：COVID-19感染; 妊娠; 血浆/细胞外囊泡; 促炎细胞因子谱型

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    Copyright © American journal of reproductive immunology (New York, N.Y. : 1989). 中文内容为AI机器翻译，仅供参考！

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    期刊名：American journal of reproductive immunology

    缩写：AM J REPROD IMMUNOL

    ISSN：1046-7408

    e-ISSN：1600-0897

    IF/分区：2.5/Q3

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