Duck Tembusu virus (DTMUV) is an acute avian flavivirus that primarily infects poultry, mosquitoes, and some mammals including humans. The viral infection triggers reactive oxygen species (ROS) and inflammatory response that are crucial in mediating injury. Crafting multifunctional nanozymes that possess both ROS scavenging and anti-inflammatory activities presents formidable challenges. The study synthesized manganese dioxide cauliflowers (MnO₂ Cfs) endowed with multiple enzyme-like activities (analogous to SOD, CAT, and GPX) that effectively alleviated the injury induced by DTMUV both in vitro and in vivo. Remarkably, MnO₂ Cfs efficiently neutralized various ROS, encompassing hydrogen peroxide (H₂O₂), superoxide anion (O₂^·-) and hydroxyl radical (·OH). Our in vitro assessments showed that MnO₂ Cfs could alleviate cytopathic effects and modulate the innate immune response during DTMUV infection through their ROS scavenging and anti-inflammatory properties. In vivo experiments supported these findings, demonstrating that ducklings therapied by MnO₂ Cfs experienced alleviated injury during DTMUV infection. Importantly, MnO₂ Cfs also effectively inhibited DTMUV replication in both laboratory and field conditions. This study presents a novel strategy for nanozyme design, promising significant therapeutic potential for treating viral inflammatory diseases.

Keywords: Antiviral function; Avian flavivirus; Inflammatory injury; MnO(2) nanozyme; Therapy.

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鸭坦布苏病毒（DTMUV）是一种急性鸟类黄病毒，主要感染家禽、蚊子和一些包括人类在内的哺乳动物。病毒感染会引发活性氧（ROS）和炎症反应，这对损伤的介导至关重要。设计同时具备清除ROS和抗炎功能的多功能纳米酶面临巨大挑战。本研究合成了具有多种类似酶活性（类SOD、CAT和GPX）的二氧化锰花椰菜状结构（MnO₂Cfs），这些结构在体外和体内均有效缓解了DTMUV引起的损伤。值得注意的是，MnO₂Cfs能够高效中和各种ROS，包括过氧化氢（H₂O₂）、超氧阴离子（O₂^·-）和羟基自由基（·OH）。我们的体外评估显示，MnO₂Cfs可通过其清除ROS和抗炎作用减轻细胞病变效应并调节DTMUV感染期间的先天免疫反应。体内实验也支持了这些发现，证明接受MnO₂Cfs治疗的小鸭在DTMUV感染过程中经历损伤缓解。重要的是，MnO₂Cfs还在实验室和现场条件下有效抑制了DTMUV复制。这项研究为纳米酶设计提供了一种新的策略，并具有显著的治疗病毒性炎症疾病的潜力。

关键词：抗病毒功能；鸟类黄病毒；炎症损伤；MnO₂ 纳米酶；治疗。

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