As has recently been shown, Taylor-Aris dispersion-assisted mass spectrometry (TADA-MS) can offer direct injection MS determinations in fields where the targets of the analyses are large molecules present in a matrix that would otherwise cause serious interferences. In the present study, we demonstrated the exceptional utility of TADA-MS in native protein analysis: (i) a dramatic improvement in detection sensitivity was found due to its ability to strongly reduce matrix interferences, (ii) more "native-like" conditions can be used during analyses, (iii) the direct injection of non-MS-compatible matrices is allowed into MS, and (iv) a considerable simplification and economization of the workflow is ensured. We investigated the behavior of different types of proteins and protein complexes present under native conditions, demonstrating the unambiguous benefits and simplicity of the method.
Analytical chemistry. 2024 Jul 16;96(28):11309-11317. doi: 10.1021/acs.analchem.4c01270 Q16.82024
Taylor-Aris Dispersion-Assisted Mass Spectrometry for the Analysis of Native Proteins
泰勒-阿里斯色散增强型质谱法用于分析天然蛋白质 翻译改进
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DOI: 10.1021/acs.analchem.4c01270 PMID: 38946421
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