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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2022 Nov 1:1210:123477. doi: 10.1016/j.jchromb.2022.123477 Q22.82025

Oral fluid as a new investigative matrix for the determination of organic gunshot residue exposure

口腔液体作为测定有机火药残留物的新检测介质 исследование 翻译改进

Flavia Pagano  1, Flaminia Vincenti  1, Camilla Montesano  2, Federico Fanti  3, Adolfo Gregori  4, Roberta Curini  5, Manuel Sergi  3

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作者单位

  • 1 Public Health and Infectious Disease Department, University La Sapienza, Rome, Italy; Chemistry Department, University La Sapienza, Rome, Italy.
  • 2 Chemistry Department, University La Sapienza, Rome, Italy. Electronic address: camilla.montesano@uniroma1.it.
  • 3 University of Teramo, Faculty of Bioscience and Technology for Food, Agriculture and Environment, Teramo, Italy.
  • 4 Carabinieri, Department of Scientific Investigation (RIS), Rome, Italy.
  • 5 Chemistry Department, University La Sapienza, Rome, Italy.

    • DOI: 10.1016/j.jchromb.2022.123477 PMID: 36174265

    摘要 Ai翻译

    In recent years, increased use of ammunition without lead and heavy metals was observed, leading to a growing interest in the detection of organic gunshot residues (OGSR) as evidence of firearms related crimes. The wide range of compounds belonging to the OGSR class hinders their mass spectrometric detection as different ionization techniques may be needed to obtain good results for all compounds. The purpose of this work was the development of a reliable analytical method by means of UHPLC-HRMS for the determination in oral fluid (OF) of the most common explosives and the most used stabilizers, arising from fire discharge and post-deflagration residues. For this purpose, SPE was used for OF clean-up before UHPLC-HRMS analysis. All target analytes were chromatographically separated by means of a Polar-C18 column. A chlorinated compound was added to the mobile phases in order to promote the formation of chloride adduct ions in the electrospray ion source operating in polarity switching to allow the best conditions for each analyte. The detection was conducted by means of a high-resolution mass spectrometer equipped with Orbitrap technology working in data dependent acquisition mode, in order to detect both the precursor ions and/or the most intense fragments for stabilizers. To verify its potential, the method was tested on real samples: a shooting session was performed in an open shooting range; the shooters fired from 2 to 20 rounds with a 9x21 caliber, thereafter OF was sampled. Samples were analyzed confirming that explosives may be detected in OF; the use of this matrix may be of great interest for investigative purposes as it is less affected by secondary transfer when compared to other commonly sampled matrices. The developed method could be a useful tool for law enforcement authorities for the detection of explosives in forensic potential scenarios, including biological matrices.

    Keywords: Forensic investigation; LC-HRMS; Oral fluid; Organic gunshot residue; Solid phase extraction.

    Keywords：oral fluid; determination; organic gunshot residue

    关键词：口腔液体; 测定; 有机枪击残留物

    Copyright © Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 中文内容为AI机器翻译，仅供参考！

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    期刊名：Journal of chromatography b-analytical technologies in the biomedical and life sciences

    缩写：J CHROMATOGR B

    ISSN：1570-0232

    e-ISSN：1873-376X

    IF/分区：2.8/Q2

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