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bioRxiv : the preprint server for biology. 2021 Jan 19:2021.01.15.426463. doi: 10.1101/2021.01.15.426463

Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine

表达于ChAdOx1 nCoV-19/AZD1222疫苗中的类似天然状态的SARS-CoV-2刺突糖蛋白 翻译改进

Yasunori Watanabe  1  2, Luiza Mendonça  3, Elizabeth R Allen  4, Andrew Howe  5, Mercede Lee  4, Joel D Allen  1, Himanshi Chawla  1, David Pulido  4, Francesca Donnellan  4, Hannah Davies  4, Marta Ulaszewska  4, Sandra Belij-Rammerstorfer  4  6, Susan Morris  4, Anna-Sophia Krebs  3, Wanwisa Dejnirattisai  7, Juthathip Mongkolsapaya  7  8  9, Piyada Supasa  7, Gavin R Screaton  7  10, Catherine M Green  7, Teresa Lambe  4  6, Peijun Zhang  3  5, Sarah C Gilbert  4  6, Max Crispin  1

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作者单位

  • 1 School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
  • 2 Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
  • 3 Division of Structural Biology, University of Oxford, Wellcome Centre for Human Genetics, Oxford, OX3 7BN, UK.
  • 4 The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • 5 Electron Bio-imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 0DE, UK.
  • 6 NIHR Oxford Biomedical Research Centre, Oxford, UK.
  • 7 The Wellcome Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.
  • 8 Dengue Hemorrhagic Fever Research Unit, Office for Research and Development, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
  • 9 Chinese Academy of Medical Science(CAMS) Oxford Institute (COI), University of Oxford, Oxford, U.K.
  • 10 Division of Medical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK.

    • DOI: 10.1101/2021.01.15.426463 PMID: 33501433

    摘要 Ai翻译

    Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibodies that they produce appropriately processed and assembled viral antigen that mimics that observed on the SARS-CoV-2 virus. Here, we describe the structure, conformation and glycosylation of the S protein derived from the adenovirus-vectored ChAdOx1 nCoV-19/AZD1222 vaccine. We demonstrate native-like post-translational processing and assembly, and reveal the expression of S proteins on the surface of cells adopting the trimeric prefusion conformation. The data presented here confirms the use of ChAdOx1 adenovirus vectors as a leading platform technology for SARS-CoV-2 vaccines.

    Keywords：SARS-CoV-2 spike glycoprotein

    关键词：SARS-CoV-2刺突糖蛋白

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