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OncoTargets and therapy. 2020 Dec 18:13:12987-12997. doi: 10.2147/OTT.S281962 Q22.72024

MiR-519d and miR-328-3p Combinatorially Suppress Breast Cancer Progression

miR-519d和miR-328-3p联合抑制乳腺癌进展 翻译改进

Haiming Ma  1, Tao Liu  2, Yanhua Xu  3, Xinying Wang  4, Jin Wang  1, Xiaokang Liu  1

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作者单位

  • 1 Department of Oncology, Guangrao County People's Hospital, Dongying City, Shandong Province 257300, People's Republic of China.
  • 2 Department of General Surgery, Guangrao County People's Hospital, Dongying City, Shandong Province 257300, People's Republic of China.
  • 3 Department of Anesthesiology, Guangrao County People's Hospital, Dongying City, Shandong Province 257300, People's Republic of China.
  • 4 Department of Pathology, Guangrao County People's Hospital, Dongying City, Shandong Province 257300, People's Republic of China.
  • DOI: 10.2147/OTT.S281962 PMID: 33376348

    摘要 Ai翻译

    Background: MiR-519d and miR-328-3p had tumor-regulatory properties in different cancers, but their combinatorial effects and potential common target in breast cancer had not been fully reported. This research targeted to study the underlying mechanism of how miR-519d and miR-328-3p cooperatively suppressed breast cancer.

    Methods: MiR-519d and miR-328-3p expressions in breast cancer tissues and cells were assessed and Ki-67 expression was also checked. DLR assay was executed to verify whether Ki-67 was a common target of miR-519d and miR-328-3p. Western blot, flow cytometry, colony formation, wound healing and transwell assays were applied to examine the inhibitory roles of these two miRNAs on the malignant behaviors of breast cancer cells and the potential molecular mechanism.

    Results: Impeded miR-519d and miR-328-3p expressions and enhanced Ki-67 expression were detected in breast cancer tissues and cells. Ki-67 was confirmed as a target of these two miRNAs. MiR-519d and miR-328-3p hampered cell proliferation and blocked cell cycle via binding to Ki-67 and they also suppressed migration and invasion. The combinatorial effects of two miRNAs were much stronger than a single miRNA.

    Conclusion: Our findings proved that miR-519d and miR-328-3p played combinatorial anti-cancer roles in breast cancer by directly targeting a common target Ki-67. Our study suggested that these two miRNAs might own the potential to become novel therapeutic biomarkers involved in the diagnosis and therapy of breast cancer.

    Keywords: Ki-67; breast cancer; miR-328-3p; miR-519d.

    Keywords:breast cancer; progression

    关键词:乳腺癌; 进展

    Copyright © OncoTargets and therapy. 中文内容为AI机器翻译,仅供参考!

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    期刊名:Oncotargets and therapy

    缩写:ONCOTARGETS THER

    ISSN:1178-6930

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    IF/分区:2.7/Q2

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    MiR-519d and miR-328-3p Combinatorially Suppress Breast Cancer Progression