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Insect biochemistry and molecular biology. 2008 Oct;38(10):950-8. doi: 10.1016/j.ibmb.2008.07.009 Q13.72025

Proteomic analysis of the peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera

军刀夜盗蛾幼虫肠道半透屏障的蛋白质组分析 翻译改进

Peter M Campbell  1, Anh T Cao, Eric R Hines, Peter D East, Karl H J Gordon

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  • 1 CSIRO Entomology, Canberra, Australia. peter.m.campbell@csiro.au

    • DOI: 10.1016/j.ibmb.2008.07.009 PMID: 18760362

    摘要 Ai翻译

    The peritrophic matrix from the midgut of the caterpillar, Helicovera armigera, was solubilized by treatment with anhydrous trifluoromethanesulfonic acid, apparently by depolymerisation of its chitin component. This allowed the efficient extraction of proteins in a technique that may be broadly applicable to the analysis of other structures containing chitin. Gel electrophoresis and mass spectrometry of tryptic peptides were used to identify the extracted ... ...点击完成人机验证后继续浏览
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    期刊名：Insect biochemistry and molecular biology

    缩写：INSECT BIOCHEM MOLEC

    ISSN：0965-1748

    e-ISSN：1879-0240

    IF/分区：3.7/Q1

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    Proteomic analysis of the peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera