Pepsin-solubilized collagen (PSC) was conjugated with carboxymethyl dextran (CMD) using cyanogen bromide to obtain a PSC-CMD film having improved physical properties, physiological properties, and cell affinity. The conjugation was confirmed by the loss of the alpha- and beta-subunit chains and the polymerized band on SDS-PAGE, and by a decrease in the isoelectric point to 3.2. PSC-CMD had a large polymerized structure with the 6 PSC and 228 CMD molecules. PSC-CMD was readily soluble in water, reconstructed a matrix with a less-ordered structure and a characteristic morphological shape, and lost platelet aggregation-inducing ability. The PSC-CMD film, cross-linked by ultraviolet irradiation, exhibited reduced solubility, moderate water vapor permeability, and increased flexibility. PSC-CMD coatings exhibited good cell attachment and growth for fibroblasts and vein endothrical cells.
Bioscience, biotechnology, and biochemistry. 2008 Jun;72(6):1438-47. doi: 10.1271/bbb.70751 Q41.42024
Polymerized structure and improved physical and biochemical material properties of carboxymethyl dextran-conjugated collagen
羧甲基右旋糖酐缀合胶原的聚合结构及其物理和生物化学性能改善 翻译改进
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DOI: 10.1271/bbb.70751 PMID: 18540095
摘要 Ai翻译
Keywords：physical properties; biochemical properties
关键词：羧甲基葡聚糖共轭胶原; 物理性质; 生化性质
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