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Oxidation of the guanosine moiety in DNA has become a hallmark biomarker in assessing oxidative stress. The oxidation of guanosine in the nucleotide triphosphate pool has been overlooked due to the lack of a reliable methodology. This method describes a sample processing and high performance liquid chromatography with electrochemical detection protocol for the analysis of the cellular pool of guanosine triphosphates and oxidized guanosine triphosphates. Validation of this method is demonstrated along with evaluation of these analytes in control and oxidizing conditions in vitro and in HEK 293T cells. Oxidation of this triphosphate pool occurred independently of oxidation to DNA.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2007 Sep 1;856(1-2):121-30. doi: 10.1016/j.jchromb.2007.05.034 Q32.82024
Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection
高效液相电化学检测法分析鸟苷和氧化型核苷三磷酸酯以评估氧化应激标志物 翻译改进
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DOI: 10.1016/j.jchromb.2007.05.034 PMID: 17581804
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Keywords：oxidative stress; biomarkers
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期刊名：Journal of chromatography b-analytical technologies in the biomedical and life sciences
缩写：J CHROMATOGR B
ISSN：1570-0232
e-ISSN：1873-376X
IF/分区：2.8/Q3
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Assessing biomarkers of oxidative stress: analysis of guanosine and oxidized guanosine nucleotide triphosphates by high performance liquid chromatography with electrochemical detection