Several HPLC methods with UV detection were developed for qualitative and quantitative analysis of chlorin e(6) and photosensitizer Photolon either in the free form or upon pre-derivatization (methylation) under reversed- and normal-phase conditions. Optimum analysis conditions providing the best resolution of analytes were found at acidic pH where polar groups are completely protonated. The separation was performed by gradient elution with mobile phases of 0.08% trifluoroacetic acid and acetonitrile on an XTerra RP(18) column. The method was specific, accurate and precise, allowing the analysis of chlorin e(6) in the presence of numerous degradation products useful in the manufacturing process and quality control of chlorin e(6) and Photolon.
Biomedical chromatography : BMC. 2007 Mar;21(3):318-25. doi: 10.1002/bmc.762 Q31.72024
HPLC study of chlorin e6 and its molecular complex with polyvinylpyrrolidone
高效液相色谱法研究氯霉素e6及其与聚乙烯吡咯酮的分子复合物 翻译改进
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DOI: 10.1002/bmc.762 PMID: 17236242
摘要 Ai翻译
Keywords：chlorin e6; polyvinylpyrrolidone
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