A Fluorescent Sex-Sorting Technique for Insects with the Demonstration in Drosophila melanogaster [0.03%]
一种适用于昆虫的荧光性别分选技术在黑腹果蝇中的应用示范
Junru Liu,Danny Rayes,Omar S Akbari
Junru Liu
Recent advances in insect genetic engineering offer alternative genetic biocontrol solutions to control populations of pests and disease vectors. While success has been achieved, sex-sorting remains problematic for scaling many genetic bioc...
Optimization of Nuclear Localization Signal Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells [0.03%]
优化核定位信号组成可提高CRISPR-Cas12a在人类原代细胞中的编辑效率
Kevin Luk,Pengpeng Liu,Jing Zeng et al.
Kevin Luk et al.
Type V CRISPR-Cas12a systems are an attractive Cas9-alternative nuclease platform for specific genome editing applications. However, previous studies demonstrate that there is a gap in overall activity between Cas12a and Cas9 in primary cel...
Regulating the Clock: REV-ERB Agonists as Promising Therapeutic Agents [0.03%]
调控生物钟： REV-ERB 激动剂作为有潜力的治疗手段
Chandrashekhar Prasad,Isaac Kirubakaran Sundar
Chandrashekhar Prasad
Engineering T Cell Development for the Next Generation of Stem Cell-Derived Immunotherapies [0.03%]
工程化T细胞的发育以用于下一代干细胞衍生免疫疗法
Yale S Michaels,Lauren J Durland,Peter W Zandstra
Yale S Michaels
Engineered T cells are at the leading edge of clinical cell therapy. T cell therapies have had a remarkable impact on patient care for a subset of hematological malignancies. This foundation has motivated the development of off-the-shelf en...
A Genome-Wide RNAi Screen Reveals Common Host-Virus Gene Signatures: Implication for Dengue Antiviral Drug Discovery [0.03%]
全基因组RNAi筛选发现宿主-病毒共同基因特征：登革热抗病毒药物研究的启示
David Shum,Bhavneet Bhinder,Jeni Mahida et al.
David Shum et al.
Dengue is the most common mosquito-borne viral disease that in recent years has become a major international public health concern. Dengue is a tropical neglected disease with increasing global incidences, affecting millions of people world...
Synthetic Reader-Actuators Targeted to Polycomb-Silenced Genes Block Triple-Negative Breast Cancer Proliferation and Invasion [0.03%]
合成型读取器致动器靶向多梳沉默基因可抑制三阴性乳腺癌增殖和侵袭
Lauren Hong,Natecia L Williams,Maya Jaffe et al.
Lauren Hong et al.
Scientists have used pharmacological inhibitors of polycomb proteins to restore the expression of tumor suppressor genes and stop cancer proliferation and invasion. A major limitation of this approach is that key transcriptional activators,...
Generative Artificial Intelligence-Assisted Protein Design Must Consider Repurposing Potential [0.03%]
生成式人工智能辅助的蛋白质设计必须考虑再利用潜力
Sean Ekins,Maximilian Brackmann,Cédric Invernizzi et al.
Sean Ekins et al.
Generative artificial intelligence software used for chemical and protein design has repurposing potential. We propose careful discussion in the biotech community on security considerations of such technologies and serious consideration of ...
Comparative Evaluation of Synthetic Cytokines for Enhancing Production and Performance of NK92 Cell-Based Therapies [0.03%]
用于增强NK92细胞治疗产品生产和性能的合成细胞因子效果比较评估
Simrita Deol,Patrick S Donahue,Roxana E Mitrut et al.
Simrita Deol et al.
Off-the shelf immune cell therapies are potentially curative and may offer cost and manufacturing advantages over autologous products, but further development is needed. The NK92 cell line has a natural killer-like phenotype, has efficacy i...
Bioorthogonal Stimulated Raman Scattering Imaging Uncovers Lipid Metabolic Dynamics in Drosophila Brain During Aging [0.03%]
生物正交刺激拉曼散射成像揭秘果蝇大脑在衰老过程中的脂质代谢动力学现象
Yajuan Li,Phyllis Chang,Shriya Sankaran et al.
Yajuan Li et al.
Studies have shown that brain lipid metabolism is associated with biological aging and influenced by dietary and genetic manipulations; however, the underlying mechanisms are elusive. High-resolution imaging techniques propose a novel and p...
Axl and Vascular Endothelial Growth Factor Receptors Exhibit Variations in Membrane Localization and Heterogeneity Across Monolayer and Spheroid High-Grade Serous Ovarian Cancer Models [0.03%]
Axl和血管内皮生长因子受体在单层和球形高级别浆液性卵巢癌模型中的膜定位和异质性差异分析
Yingye Fang,Princess I Imoukhuede
Yingye Fang
Vascular endothelial growth factor receptors (VEGFRs) and Axl are receptor tyrosine kinases (RTK) that are targeted in ovarian cancer therapy. Two-dimensional monolayer culture and three-dimensional spheroids are common models for RTK-targe...