Practical considerations for machine learning-enabled discoveries in spatial transcriptomics [0.03%]
空间转录组学中机器学习驱动发现的实践思考
Alex J Lee,Robert Cahill,Reza Abbasi-Asl
Alex J Lee
Development and homeostasis in multicellular systems require exquisite control over spatial molecular pattern formation and maintenance. Advances in spatially-resolved and high-throughput molecular imaging methods such as multiplexed immuno...
Long-Term Suppression of RUNX2 During MSC-Based Cartilage Formation Increases Cartilage Matrix Accumulation [0.03%]
RUNX2的长期抑制可增加基于间充质干细胞的软骨形成中的软骨基质积累
Ruxin Yang,Tiana J Wong,Rhima M Coleman
Ruxin Yang
The objective of this study was to evaluate the long-term effects of an autonomous RUNX2-supressing gene circuit on cartilage matrix accumulation and compressive mechanics in the tissues produced by MSC-derived chondrocytes (MdChs). Human m...
Ana C Puhl,Sarah Negri,Maggie A Z Hupcey et al.
Ana C Puhl et al.
There are thousands of rare genetic diseases lacking an approved treatment, many of which are life-limiting to children. Those caused by a missing protein may represent a target for protein replacement either by enzyme replacement therapy o...
Exploring structure-function relationships in engineered receptor performance using computational structure prediction [0.03%]
利用计算结构预测研究工程受体性能中的结构功能关系
William K Corcoran,Amparo Cosio,Hailey I Edelstein et al.
William K Corcoran et al.
Engineered receptors play increasingly important roles in transformative cell-based therapies. However, the structural mechanisms that drive differences in performance across receptor designs are often poorly understood. Recent advances in ...
Revolutionizing Postdoctoral Training Using the Social Ecological Model: Insights and Experiences from the Propel Scholars [0.03%]
基于社会生态模型改造博士后培训模式：Propel学者项目的启示与经验
Cellas A Hayes,Colwyn A Headley,Amy R Nava et al.
Cellas A Hayes et al.
The dissatisfaction within the postdoctoral training phase has led to the drastic reduction in the number of U.S. citizens pursuing postdoctoral positions within the biological and biomedical sciences fields. Even more so, there is an obvio...
Real-Time Monitoring in Biomanufacturing with Graphene Field-Effect Transistor Sensors: Detection of pH, Glucose, and Antibodies [0.03%]
石墨烯场效应晶体管传感器在生物制造中的实时监测：检测pH值、葡萄糖和抗体
Deependra K Ban,Reza Hajian,Matthew Chan et al.
Deependra K Ban et al.
In high-efficiency smart biomanufacturing, continuous monitoring of products, byproducts, and reagents is crucial for optimizing production processes, improving yield, and ensuring product quality. This monitoring maintains optimal conditio...
In Vivo Prime Editing by Lipid Nanoparticle Co-delivery of Chemically Modified pegRNA and Prime Editor mRNA [0.03%]
基于脂质纳米粒共递送化学修饰pegRNA和Prime Editor mRNA的体内先导编辑技术
Zexiang Chen,Karen Kelly,Haoyang Cheng et al.
Zexiang Chen et al.
Prime editing has gained significant attention as a next-generation gene editing technology, owing to its unique advantages. However, realizing its potential in vivo requires effective delivery strategies. While adeno-associated virus (AAV)...
Guohao Liang,Hong Yin,Fangyuan Ding
Guohao Liang
Transcriptomics is one of the largest areas of research in biological sciences. Aside from RNA expression levels, the significance of RNA spatial context has also been unveiled in the recent decade, playing a critical role in diverse biolog...
Improved cell-type identification and comprehensive mapping of regulatory features with spatial epigenomics 96-channel microfluidic platform [0.03%]
改进的细胞类型识别和使用空间表观遗传学96通道微流控平台调节特征的全面图谱绘制
Zev Kartiganer,Gumaro Rojas,Machele Riccio et al.
Zev Kartiganer et al.
Gene expression is subject to epigenetic regulation and is dependent upon cellular context. Spatial omics tools can provide insight into cellular context; however, development has centered on spatial transcriptomics and proteomics. Determin...
Influence of Alzheimer's disease related neuropathology on local microenvironment gene expression in the human inferior temporal cortex [0.03%]
阿尔茨海默病相关神经病理学对人类颞下皮层局部微环境基因表达的影响
Sang Ho Kwon,Sowmya Parthiban,Madhavi Tippani et al.
Sang Ho Kwon et al.
Neuropathological lesions in the brains of individuals affected with neurodegenerative disorders are hypothesized to trigger molecular and cellular processes that disturb homeostasis of local microenvironments. Here, we applied the 10x Geno...