The development of a direct co-culture-based model for diabetic foot ulcer mimicking inflammation and impaired phagocytosis [0.03%]
一种模拟糖尿病足溃疡炎症和吞噬作用受损的直接共培养模型的发展
Mirella Ejiugwo,Yury Rochev,Georgina Gethin et al.
Mirella Ejiugwo et al.
Purpose: Diabetic foot ulcers (DFU) are characterized by delayed healing and high infection rates. DFU affect approximately 25% of individuals with diabetes. Secondary to hyperglycaemia, both chronic inflammation and defe...
Mechanical scratch injury on differentiated motor neuron of NSC-34 cells as an in vitro model for evaluation of neuroregeneration potential of NeuroAiD II (MLC901) [0.03%]
NSC-34细胞分化运动神经元的机械划痕损伤作为体外模型评估NeuroAid II（MLC901）神经再生潜能
Anam Anjum,Muhammad Dain Yazid,Muhammad Fauzi Daud et al.
Anam Anjum et al.
Background: Spinal cord regeneration is considered an ultimate achievement in the field of neuroscience. In vitro, neural stem cell (NSC-34) motor neuron-like cell cultures are powerful tools to study specific molecular p...
A comparative analysis of vitrification and two slow freezing methods for gonocyte-containing neonatal calf testicular tissue and subsequent in vitro culture [0.03%]
玻璃化和两种慢速冷冻方法对含有生精细胞的新生小牛睾丸组织的比较分析及其体外培养后续步骤
Shiyan Tang,Celine Jones,Jill Davies et al.
Shiyan Tang et al.
The cryopreservation of neonatal testicular tissue containing gonocytes is crucial for preserving genetic diversity, advancing research, and developing reproductive technologies. In this study, we investigated three cryopreservation techniq...
Colorectal carcinoma organoid and cancer-associated fibroblasts co-culture system for drug evaluation [0.03%]
结直肠癌类器官与癌相关成纤维细胞共培养系统在药物评估中的应用
Yan Wang,Zilin Zhang,Xiaoran Li et al.
Yan Wang et al.
Patient-derived organoids (PDO) have the potential to be used as preclinical cancer models for testing anti-cancer drug efficiency. Cancer-associated fibroblasts (CAFs), which have been closely linked with colorectal carcinoma (CRC) progres...
An in vitro model to measure the strength and stiffness of the extracellular matrix synthesized de novo by human fibroblasts [0.03%]
一种测量人类成纤维细胞从头合成的细胞外基质的力量和刚度的体外模型
Yanying Wu,Jayla Millender,Becka Padgett et al.
Yanying Wu et al.
Purpose: Alterations to the strength and stiffness of the human extracellular matrix (ECM) are the underlying pathology manifest in a wide range of diseases. These include inherited conditions, such as Ehlers Danlos syndr...
A standardized in vitro bioengineered skin for penetrating wound modeling [0.03%]
用于穿透性伤口建模的标准体外生物工程皮肤
C Sofia Salazar Silva,Werner Petzold,Ulrike Hirsch et al.
C Sofia Salazar Silva et al.
Over the years, significant progress has been made in developing cost-effective and ethical in vitro bioengineered skin substitutes to study cutaneous wound healing processes. Rodents and small animal models are not optimal due to physiolog...
Organoids meet microfluidics: recent advancements, challenges, and future of organoids-on-chip [0.03%]
器官芯片中类器官的应用：最新进展、挑战及前景
Talha Chauhdari,Syeda Armana Zaidi,Jilei Su et al.
Talha Chauhdari et al.
Organoids are three-dimensional, miniaturized tissue-like structures derived from either stem cells or primary cells, emerging as powerful in vitro models for studying developmental biology, disease pathology, and drug discovery. These orga...
Pancreatic cancer extracellular vesicles stimulate Schwann cell activation and perineural invasion in vitro via IL-8/CCL2 [0.03%]
胰腺癌细胞外囊泡通过IL-8/CCL2促进雪旺氏细胞活化及体外神经周浸润
Emory Gregory,Isabel Powers,Azemat Jamshidi-Parsian et al.
Emory Gregory et al.
Purpose: Pancreatic ductal adenocarcinoma (PDAC) remains a leading cause of cancer-related deaths, and perineural invasion (PNI), in which cancer cells infiltrate nerves, enables metastasis in most patients. PNI is largel...
Biological characterization of breast cancer spheroid formed by fast fabrication method [0.03%]
快速制备方法构建的乳腺癌类器官体的生物学性质研究
Yuta Iijima,Norino Uenaka,Mayu Morimoto et al.
Yuta Iijima et al.
Engineered three-dimensional (3D) tissue culture platforms are useful for reproducing and elucidating complex in vivo biological phenomena. Spheroids, 3D aggregates of living cells, are produced based on physicochemical or microfabrication ...
Navigating the neuroinflammatory network: insights from diverse cell models [0.03%]
导航神经炎症网络：来自多种细胞模型的见解
Libia Catalina Salinas Castellanos,Mayra Micaela Montes,Georgina Oriana Mingolo Malnati et al.
Libia Catalina Salinas Castellanos et al.
In this Highlights article, we present insights into the use of simple cell lines in neuroinflammation research, highlighting key findings from our recent investigations. Simple cell lines, including HEK, PC12, SHSY5Y, and N2a cells, provid...