In vitro cancer models as an approach to identify targetable developmental phenotypes in cancer stem cells [0.03%]
体外癌症模型在识别癌干细胞中可靶向的发育表型方面的应用
Adrian Biddle
Adrian Biddle
Cancer therapeutics are often highly toxic to the patient, and they often elicit rapid resistance in the tumour. Recent advances have suggested a potential new way in which we may improve on this, through two important concepts: (1) that mu...
Application of a 3D hydrogel-based model to replace use of animals for passaging patient-derived xenografts [0.03%]
三维水凝胶模型在人源异种移植物传代动物实验替代中的应用
Sal Jones,Jennifer C Ashworth,Marian Meakin et al.
Sal Jones et al.
Purpose: This 3D in vitro cancer model for propagation of patient-derived cells, using a synthetic self-assembling peptide gel, allows the formation of a fully characterised, tailorable tumour microenvironment. Unlike man...
Urgent action is required to increase sustainability in in vitro modelling [0.03%]
亟需采取行动以提高体外模型的可持续性
Katarina Pedersen,Anne Vanhoestenberghe,Susan Heavey
Katarina Pedersen
Inflammatory bowel disease addressed by Caco-2 and monocyte-derived macrophages: an opportunity for an in vitro drug screening assay [0.03%]
人结肠癌Caco-2细胞和单核细胞源性巨噬细胞对炎症性肠病的体外药物筛选模型价值研究
Sabrina Schnur,Vanessa Wahl,Julia K Metz et al.
Sabrina Schnur et al.
Inflammatory bowel disease (IBD) is a widespread disease, affecting a growing demographic. The treatment of chronic inflammation located in the GI-tract is dependent on the severity; therefore, the IBD treatment pyramid is commonly applied....
A novel colorimetric biosensor for detecting SARS-CoV-2 by utilizing the interaction between nucleocapsid antibody and spike proteins [0.03%]
一种利用核衣壳抗体和刺突蛋白相互作用检测SARS-CoV-2的新型比色生物传感器
Abhishek Bhattacharjee,Roberta M Sabino,Justin Gangwish et al.
Abhishek Bhattacharjee et al.
SARS-CoV-2 is a pandemic coronavirus that causes severe respiratory disease (COVID-19) in humans and is responsible for millions of deaths around the world since early 2020. The virus affects the human respiratory cells through its spike (S...
Abhishek Joshi,Arun Soni,Sanjeev Acharya
Abhishek Joshi
Inflammatory bowel disease (IBD) is a chronic, relapsing gastrointestinal condition. Ulcerative colitis and Crohn's disease are types of inflammatory bowel disease. Over many decades, the disease has been a topic of study, with experts stil...
3D spheroid culture to examine adaptive therapy response in invading tumor cells [0.03%]
三维球状培养技术检测侵入肿瘤细胞的适应性疗法反应
Felix Weiss,Nader Atlasy,Vince van Reijmersdal et al.
Felix Weiss et al.
3D in vitro culture models of cancer cells in extracellular matrix (ECM) have been developed to investigate drug targeting and resistance or, alternatively, mechanisms of invasion; however, models allowing analysis of shared pathways mediat...
Development and evaluation of a bovine lung-on-chip (bLOC) to study bovine respiratory diseases [0.03%]
牛肺芯片(bLOC)的开发与评估，用于研究牛呼吸系统疾病
Diane F Lee,Clare L Thompson,Ronald E Baynes et al.
Diane F Lee et al.
Purpose: Current air-liquid interface (ALI) models of bovine proximal airways have their limitations. They do not simulate blood flow necessary to mimic systemic drug administration, and repeated sampling requires multipl...
Oscillations of the circadian clock protein, BMAL-1, align to daily cycles of mechanical stimuli: a novel means to integrate biological time within predictive in vitro model systems [0.03%]
昼夜节律钟蛋白BMAL-1的振荡与每日机械刺激周期对齐：在体外模型系统中整合生物时间的一种新方法
Hannah K Heywood,Laurence Gardner,Martin M Knight et al.
Hannah K Heywood et al.
Purpose: In vivo, the circadian clock drives 24-h rhythms in human physiology. Isolated cells in vitro retain a functional clockwork but lack necessary timing cues resulting in the rapid loss of tissue-level circadian rhy...
Novel application of live imaging to determine the functional cell biology of endothelial-to-mesenchymal transition (EndMT) within a liver-on-a-chip platform [0.03%]
利用活体成像技术确定芯片上的肝脏平台内内皮细胞到间充质转化（EndMT）的功能细胞生物学的新应用
James Whiteford,Samantha Arokiasamy,Clare L Thompson et al.
James Whiteford et al.
Objective: Imaging endothelial cell behaviour under physiological conditions, particularly those associated with chronic fibrotic pathologies, is an incredibly challenging endeavour. While short-term assessments (hours) c...