A flow cytometric method for measuring and isolating mammary epithelial cells from bovine milk [0.03%]
一种用于测量和分离牛乳中乳腺上皮细胞的流式细胞术方法
A J Lengi,M Makris,B A Corl
A J Lengi
Sampling frequent time points of mammary signaling pathways is not possible with tissue biopsies. We have validated a flow cytometry and cell sorting procedure for isolating live bovine mammary epithelial cells from somatic cell populations...
Rehydration characteristics of milk protein concentrate powders monitored by electrical resistance tomography [0.03%]
电阻层析成像监测的乳蛋白浓缩粉的复水特性研究
K S Babu,J K Amamcharla
K S Babu
Electrical resistance tomography (ERT) is a robust and low-cost method offering real-time visualization of processes. In this work, we developed an ERT-based method to characterize the rehydration behavior of milk protein concentrate (MPC) ...
D R Wood,R M Blome,L C Ribeiro et al.
D R Wood et al.
The objective of this trial was to compare the health and performance of calves provided high feeding rates of a whey-based all-milk-protein calf milk replacer (MR) with those fed an MR containing either 5% or 10% porcine plasma, which repl...
An alternative interpretation of residual feed intake by phenotypic recursive relationships in dairy cattle [0.03%]
通过奶牛表型递归关系对残差采食量的另一种解释
Xiao-Lin Wu,Kristen L Parker Gaddis,Javier Burchard et al.
Xiao-Lin Wu et al.
There has been increasing interest in residual feed intake (RFI) as a measure of net feed efficiency in dairy cattle. Residual feed intake phenotypes are obtained as residuals from linear regression encompassing relevant factors (i.e., ener...
Development of monoclonal antibodies for quantification of bovine tumor necrosis factor-α [0.03%]
用于定量牛肿瘤坏死因子-α的单克隆抗体的开发
Anja Sipka,Susanna Babasyan,Sabine Mann et al.
Anja Sipka et al.
The expression of the proinflammatory cytokine tumor necrosis factor-α (TNF-α) is associated with production losses in dairy cows and is a hallmark of early inflammatory processes. Reliable tools for the detection and quantification of so...
Sodium caseinate hinders chymosin-induced aggregation of caseins in concentrated milk: The role of soluble caseins and calcium ions [0.03%]
钠酪蛋白阻碍凝乳酶诱导的浓奶中酪蛋白聚集：可溶性酪蛋白和钙离子的作用
P Krishnankutty Nair,M Corredig
P Krishnankutty Nair
Concentrated casein micelle suspensions show an altered balance between the colloidal and soluble phases compared with native skim milk. The objective of this research was to probe the role of such a change on the chymosin-driven destabiliz...
Effects of weaning on regulators of volatile fatty acid absorption and intracellular pH in Holstein calves [0.03%]
断奶对荷斯坦小牛挥发性脂肪酸吸收和细胞内pH调节的影响
Rebecca L Hiltz,Dana E McCurdy,Steven Moreland et al.
Rebecca L Hiltz et al.
This study examined the effects of changes in rumen fermentation during the weaning transition on abundance of transporters involved in volatile fatty acid (VFA) absorption or intracellular pH homeostasis. Holstein bull calves (n = 27) were...
Where do we find missing data in a commercial real-time location system? Evidence from 2 dairy farms [0.03%]
商业实时定位系统中的缺失数据出现在哪里？来自2个奶牛场的证据
Keni Ren,Per Peetz Nielsen,Moudud Alam et al.
Keni Ren et al.
Real-time indoor positioning using ultra-wideband devices provides an opportunity for modern dairy farms to monitor the behavior of individual cows; however, missing data from these devices hinders reliable continuous monitoring and analysi...
Validation of an alternate method for monitoring the presence of cows at the feed bunk in a Calan Broadbent Feeding System using a 3-axis, data-logging accelerometer [0.03%]
使用三轴数据记录加速度计监测Calan Broadbent采食系统中奶牛在饲槽处活动的替代方法及其验证
Cesar Matamoros,Rebecca A Bomberger,Kevin J Harvatine
Cesar Matamoros
Daily dry matter intake is a key observation in dairy nutrition, and observation of feeding behavior provides insight into the physiological control of hunger and satiety that regulate intake. The objective of the study was to develop and v...