Detection technologies for RNA modifications and their applications in plants [0.03%]
用于植物的RNA修饰检测技术及其应用
Jiayin Du,WingTing Cheung,Lixing Zhang et al.
Jiayin Du et al.
Chemical modifications of RNA molecules play diverse regulatory roles in gene expression by influencing RNA biogenesis, stability, and translation. Emerging evidence indicates that RNA modifications in plants have functional significance fo...
Engineering high-efficiency mutants of the transposase ISDra2 TnpB via protein-generative models and energy-optimized design [0.03%]
基于蛋白质生成模型和能量优化设计的高效ISDra2转座酶TnpB突变体工程化改造
Feifei Guan,Yekun Ding,Guoshun Xu et al.
Feifei Guan et al.
Transposon-associated TnpB is a compact and versatile gene editor that holds significant promise in the life sciences. However, current engineering strategies for TnpB are limited, resulting in low cleavage efficiency in mammalian cells tha...
Xinran Zhang,Jiangbo Wei
Xinran Zhang
Chemical modifications of RNA, such as N 6-methyladenosine (m6A), have emerged as critical regulators of gene expression and chromatin dynamics in eukaryotes. Research on RNA modifications in plants has primarily focused on mRNAs and their ...
Selenium nanoparticles and glutathione synergistically enhance salt tolerance in soybean via the jasmonic acid pathway and arbutin-regulated rhizosphere microbiota [0.03%]
硒纳米粒子和谷胱甘肽通过水杨酸途径和绿原酸调节根系微生物群协同增强大豆耐盐性
Yanxi Chen,Shuai Zhao,Xuanxuan Ma et al.
Yanxi Chen et al.
Soil salinity threatens agriculture worldwide. Nano-fertilizers offer a promising strategy to enhance tolerance to salinity and other stresses in crops, but their field performance is sometimes unpredictable, potentially due to complex inte...
Enhancing the efficiency of nuclease-based prime editing in rice with the Tf1 reverse transcriptase [0.03%]
利用Tf1逆转录酶提高基于核酸酶的prime编辑在水稻中效率
Guigen Ma,Hao Xu,Sujie Zhang et al.
Guigen Ma et al.
The development of efficient and precise genome-editing tools is crucial for advancing functional genomics and improving crops. Our previously established nuclease-mediated prime editing (NM-PE) system, which combines the SpCas9 nuclease wi...
The oxidation of ABI4 by RBOHD-derived reactive oxygen species integrates redox signaling into abscisic-acid and drought-stress responses [0.03%]
RBOHD衍生的活性氧介导ABI4的氧化反应以整合植物激素ABA和干旱胁迫信号
Zhenglin Ge,Tiantian Wu,Zhengyao Lin et al.
Zhenglin Ge et al.
The phytohormone abscisic acid (ABA) induces stomatal closure and facilitates plant adaptation to drought stress. Reactive oxygen species (ROS) produced by the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN D (RBOHD) are essential ...
A class II 5-enolpyruvylshikimate-3-phosphate synthase from Pseudomonas P818 confers robust glyphosate tolerance in transgenic plants [0.03%]
来自于假单胞菌P818的二类5-烯丙基-3-磷酸 shikimate合成酶在转基因植物中赋予其良好的抗草甘膦性状
Yuhao Su,Jie Cao,Yan Liu et al.
Yuhao Su et al.
Glyphosate is a widely used herbicide that targets 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), blocking the shikimate pathway and leading to plant death. The discovery of novel EPSPS genes is key to engineering glyphosate tolerance...
Non-enzymatic acetylation of lysine residues affects the stability of Rubisco activase [0.03%]
赖氨酸残基的非酶促乙酰化影响Rubisco激活酶的稳定性
Lili Yang,Yanjun Li,Hui Hong et al.
Lili Yang et al.
Post-translational modifications of non-histone proteins are critical for plant adaptation to fluctuating environmental conditions. Rubisco activase (RCA), a chloroplastic ATPase, plays a crucial role in CO2 fixation by activating ribulose-...
The evolution of gene expression in allopolyploid cotton relative to parental diploids and induced haploids [0.03%]
异源多倍体棉花相对于亲本二倍体和诱导的单倍体的基因表达进化
Haotian Luo,Yan Li,Xianpeng Xiong et al.
Haotian Luo et al.
Allopolyploidization, a process involving interspecific hybridization and whole-genome duplication, has both immediate and long-term evolutionary effects on plants. However, the immediate impact of genome doubling in cotton (Gossypium spp.)...
Dongjiao Wang,Zhankun Li,Wentao Yang et al.
Dongjiao Wang et al.
Engineering the Arabidopsis cell-surface immune receptor RECEPTOR-LIKE PROTEIN 23 (RLP23) enhances plant immunity by enabling functional signaling triggered by the conserved pathogen peptide nlp20. Structural optimization enables broad-spec...