Improved efficiency of genetic transformation for the development of elite germplasm using CRISPR/Cas12i3 in maize [0.03%]
用于玉米优质种质开发的CRISPR/Cas12i3遗传转化效率改进方法
Yao Wang,Lu Zhang,Mengyuan Liu et al.
Yao Wang et al.
Genetic transformation and gene-editing technologies have driven progress in molecular design breeding. Jing 724, an elite founder inbred line from the X heterotic group in maize, was previously recalcitrant to genetic transformation owing ...
Muhammad Salman Iqbal,Revocatus Bahitwa,Abdul Ali Azam et al.
Muhammad Salman Iqbal et al.
Deep learning (DL) now enables the end-to-end design of protein binders-proteins that bind to specific targets-to precisely modulate protein-protein interactions (PPIs). Models and tools such as BindCraft, AlphaFold, RoseTTAFold, RFdiffusio...
Deciphering the dynamics of active autonomous terminal inverted repeat transposons in the plant kingdom [0.03%]
植物界活性自主终端重复转座子动力学的解读
Ziye Huang,Bicong Shi,Li Huang et al.
Ziye Huang et al.
Terminal inverted repeat (TIR) transposons are powerful drivers of genome evolution. However, a comprehensive understanding of their recent, lineage-specific activity across the plant kingdom has remained elusive. In this study, we develope...
Ruixiang Zhang,Zhiye Zheng,Guangzhou Li et al.
Ruixiang Zhang et al.
Base editors derived from clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) systems are widely used for genomic studies in both plants and animals. The broad applicability and safety of ba...
NLRSeek: A reannotation-based pipeline for mining missing NLR genes in sequenced genomes [0.03%]
基于重注释的挖掘基因组已测序中的缺失NLR基因的方法
Mengda Wang,Xiaowei Fu,Li Yin et al.
Mengda Wang et al.
Nucleotide-binding leucine-rich repeat (NLR) proteins function as receptors and signaling factors in plant immune systems. Identifying the genes encoding NLR proteins in genomic sequences provides crucial information for breeding disease-re...
Juan Ma,Jiangfang Qiao,Yanyong Cao et al.
Juan Ma et al.
The rhizosphere microbiome plays crucial roles in plant health by regulating nutrient cycling and enhancing stress resilience. However, due to its complexity, the rhizosphere microbiome is quite challenging to analyze using conventional app...
Mengshi Wu,Danling Zhu,Zhe Wu
Mengshi Wu
The essence of life lies in the precise regulation of genetic information flow, namely, the central dogma, with gene transcription playing a pivotal role as the starting point. For a living cell, it is not only essential via transcription t...
Multi-trait improvement of sorghum: co-expression of bar, mcry1Ab, and mvip3A enhances herbicide tolerance and pest resistance [0.03%]
多性状改良的高粱研究：Bar、mcry1Ab和mvip3A的共表达增强了对除草剂和害虫的抗性
Xuan Liu,JiangTao Yang,Yuxin Cui et al.
Xuan Liu et al.
Sorghum (Sorghum bicolor) has held significant historical importance and economic value for centuries. As consumer demands have diversified in recent years, the need for sorghum in industrial sectors such as brewing and food processing has ...
High levels of ADAR overexpression induce abundant and stochastic off-target RNA editing in rice protoplasts [0.03%]
ADAR过表达会产生大量且随机的脱靶RNA编辑效应
Shuai Yin,Shumin Li,Yang Yuan et al.
Shuai Yin et al.
Adenosine deaminase acting on RNA (ADAR) mediates adenosine-to-inosine (A-to-I) conversions, with its deaminase domain (ADARdd) acting as a core component of targeted RNA editing. However, the off-target effects of ADARdd remain poorly char...
BacPhase: Long-insert paired-end sequencing for bin marker construction and genome phasing [0.03%]
BacPhase：用于构建Bin标记和基因组分型的长插入对端序列测定法
Yinqiao Jian,Xiao Guo,Yangyang Shang et al.
Yinqiao Jian et al.
In genome sequence analysis, phasing, i.e., determining which genetic variants reside on the same chromosome, is essential for assembling genomic sequences into scaffolds. However, phasing remains challenging for plant species with autopoly...