Zhihao Hou,Wenpeng Deng,Alun Li et al.
Zhihao Hou et al.
MicroRNAs (miRNAs) and short RNA fragments (18-25 nt) are crucial biomarkers in biological research and disease diagnostics. However, their accurate and rapid detection remains a challenge, largely due to their low abundance, short length, ...
Enhanced editing efficiency in Arabidopsis with a LbCas12a variant harboring D156R and E795L mutations [0.03%]
带有D156R和E795L突变的LbCas12a变异体在拟南芥中具有增强的编辑效率
Cuiping Xin,Dexin Qiao,Junya Wang et al.
Cuiping Xin et al.
Cas12a (Cpf1), a Class 2 Type V CRISPR/Cas nuclease, has several unique attributes for genome editing and may provide a valuable alternative to Cas9. However, a low editing efficiency due to temperature sensitivity and insufficient cleavage...
Developing a CRISPR/FrCas9 system for core promoter editing in rice [0.03%]
水稻CRISPR/FrCas9核心启动子编辑系统研发
Hui Wang,Jian Ding,Jingyan Zhu et al.
Hui Wang et al.
Small mutations in the core promoter region of a gene may result in substantial changes in expression strengths. However, targeting TA-rich sequences of core promoters may pose a challenge for Cas9 variants such as SpCas9 and other G-rich P...
Regulation of gene-edited plants in Europe: from the valley of tears into the shining sun? [0.03%]
欧洲基因编辑植物监管：步入光明前的泪水山谷？
Holger Puchta
Holger Puchta
Some 20 years ago, the EU introduced complex regulatory rules for the growth of transgenic crops, which resulted in a de facto ban to grow these plants in fields within most European countries. With the rise of novel genome editing technolo...
Zhengyan Ye,Yuanyan Zhang,Shiqi He et al.
Zhengyan Ye et al.
Genome editing, particularly using the CRISPR/Cas system, has revolutionized biological research and crop improvement. Despite the widespread use of CRISPR/Cas9, it faces limitations such as PAM sequence requirements and challenges in deliv...
Efficient and precise genomic deletion in rice using enhanced prime editing [0.03%]
利用增强型prime编辑实现水稻高效精准的基因组删除
Mengyuan Liu,Xiang Zhang,Wen Xu et al.
Mengyuan Liu et al.
Efficient and precise genomic deletion shows promise for investigating the function of proteins in plant research and enhancing agricultural traits. In this study, we tested the PRIME-Del (PDel) strategy using a pair of prime editing guide ...
Li Chen,Yupeng Cai,Xiaoqian Liu et al.
Li Chen et al.
Current systems to screen for transgenic soybeans (Glycine max) involve laborious molecular assays or the expression of fluorescent markers that are difficult to see in soybean plants. Therefore, a visual system for early screening of trans...
Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot [0.03%]
优化原生质体分离和转染以及在单子叶和双子叶植物中加速Cas9/sgRNA切割效率验证的方法
Debasmita Panda,Subhasis Karmakar,Manaswini Dash et al.
Debasmita Panda et al.
The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic loci. Software-predicted guide RNAs (gRNAs) often fail to induce efficient cleavage a...
C-terminal frameshift mutations generate viable knockout mutants with developmental defects for three essential protein kinases [0.03%]
蛋白质激酶的三个本质型蛋白激酶C末端框移突变产生可以存活的敲除突变体及发育缺陷
Yun Zhang,Miao-Miao Cui,Run-Nan Ke et al.
Yun Zhang et al.
Loss-of-function mutants are fundamental resources for gene function studies. However, it is difficult to generate viable and heritable knockout mutants for essential genes. Here, we show that targeted editing of the C-terminal sequence of ...
Fusion of a rice endogenous N-methylpurine DNA glycosylase to a plant adenine base transition editor ABE8e enables A-to-K base editing in rice plants [0.03%]
将水稻内源性N-甲基嘌呤-DNA-糖基化酶融合至植物腺嘌呤碱基转换编辑器ABE8e，实现水稻中的A到K的碱基编辑
Yucai Li,Shaoya Li,Chenfei Li et al.
Yucai Li et al.
Engineering of a new type of plant base editor for simultaneous adenine transition and transversion within the editing window will greatly expand the scope and potential of base editing in directed evolution and crop improvement. Here, we i...