Application of a real-time cell analysis system in the process development and quantification of Rift Valley fever virus clone 13 [0.03%]
实时细胞分析系统在裂谷热病毒克隆13株分离和培养过程中的应用及滴度量化研究
Boitumelo Moetlhoa,Leeann Naicker,Rose Hayeshi et al.
Boitumelo Moetlhoa et al.
Conventional cell-culture viral quantification methods, namely viral plaque and 50 % tissue culture infective dose assays, are time-consuming, subjective and are not suitable for routine testing. The viral plaque formation assay is the main...
A case report: insights into reducing plastic waste in a microbiology laboratory [0.03%]
案例报告：减少微生物实验室塑料废物的思考
Joana Alves,Fiona A Sargison,Hanne Stawarz et al.
Joana Alves et al.
Single-use plastics have often replaced more sustainable materials in microbiology laboratories. Keeping in mind that one of the objectives of the United Nations Sustainable Development Goals is responsible consumption and production, we wa...
CTX-M-type ESBL-mediated resistance to third-generation cephalosporins and conjugative transfer of resistance in Gram-negative bacteria isolated from hospitals in Tamil Nadu, India [0.03%]
印度泰米尔纳德邦医院分离的革兰氏阴性菌中携带介导第三代头孢菌素耐药的CTX-M型ESBL及耐药性的接合转移
Ramesh Nachimuthu,Velu Rajesh Kannan,Bulent Bozdogan et al.
Ramesh Nachimuthu et al.
Clinical pathogens, especially Gram-negative bacteria developing resistance to third-generation cephalosporins, are making clinical outcomes more complicated and serious. This study was undertaken to evaluate the distribution of CTX-M-type ...
Corynebacterium phoceense - a rare Corynebacterium species isolated from a urine sample [0.03%]
来自尿液样本的罕见棒状杆菌科分枝菌属新种Phoceense分枝菌菌株病例报告
Claudia M Barberis,Germán M Traglia,Marisa N Almuzara et al.
Claudia M Barberis et al.
Corynebacterium spp. are Gram-positive rods that are recognized to cause opportunistic diseases under certain predisposing clinical conditions. Some species have been described in urinary tract infections. In this report we document a new e...
Case Reports
Access microbiology. 2021 Jan 27;3(2):000197. DOI:10.1099/acmi.0.000197 2021
From cloning to mutant in 5 days: rapid allelic exchange in Staphylococcus aureus [0.03%]
从克隆到突变体只需五天：金黄色葡萄球菌中的快速等位基因交换
Ian R Monk,Timothy P Stinear
Ian R Monk
In the last 10 years, the barriers preventing the uptake of foreign DNA by clinical Staphylococcus aureus isolates have been identified and powerful mutagenesis techniques such as allelic exchange are now possible in most genotypes. However...
The value of MALDI-TOF failure to provide an identification of Staphylococcal species direct from blood cultures and rule out Staphylococcus aureus bacteraemia: a post-hoc analysis of the RAPIDO trial [0.03%]
直接从血液培养瓶中鉴定葡萄球菌种的基质辅助激光解析电离飞行时间质量仪失败的价值：排除金黄色葡萄球菌败血症的一项事后分析——来自RAPIDO试验的数据
Fergus Hamilton,Rebecca Evans,Alasdair MacGowan
Fergus Hamilton
Introduction: Rapid differentiation between Staphylococcus aureus (SA) and coagulase-negative staphylococci (CoNS) is critical in clinical infection. Direct matrix-assisted laser desorption/ionization time-of-flight (MALD...
Hybrid genome assembly of Shigella sonnei reveals the novel finding of chromosomal integration of an IncFII plasmid carrying a mphA gene [0.03%]
志贺菌宋内氏球株杂交基因组组装揭示了IncFII质粒携带mphA基因整合到染色体上的新发现
Dhiviya Prabaa Muthuirulandi Sethuvel,Shalini Anandan,Dhivya Murugan et al.
Dhiviya Prabaa Muthuirulandi Sethuvel et al.
Azithromycin is increasingly being used for the treatment of shigellosis despite a lack of interpretative guidelines and with limited clinical evidence. The present study determined azithromycin susceptibility and correlated this with macro...
Microbes Against Humanity, a workshop game for horrible students: using a creative card game in higher education microbiology teaching [0.03%]
以创意卡牌游戏进行微生物教学：对抗人类的微小生物——一个面向“差生”的工作坊游戏
Georgios Efthimiou,Nicholas P Tucker
Georgios Efthimiou
Introducing creative workshops in higher education curricula, in addition to formal lectures, is an excellent way of reinforcing knowledge and encouraging creative thinking. In particular, the use of card games as a tool for inducing studen...
Case Reports
Access microbiology. 2020 Dec 3;3(2):000186. DOI:10.1099/acmi.0.000186 2020
Tsukamurella pulmonis conjunctivitis in patients with an underlying nasolacrimal duct obstruction - report of two cases [0.03%]
两例鼻泪管阻塞患者报道ツカムレラ肺菌结膜炎病例
Peter Kechker,Yigal Senderovich,Shifra Ken-Dror et al.
Peter Kechker et al.
Tsukamurella pulmonis ( Actinobacteria ), a Gram-positive, obligate aerobic and weakly or variably acid-fast bacterium, is an opportunistic pathogen. Here we report two cases of conjunctivitis caused by T. pulmonis . Both patients had a pre...
Case Reports
Access microbiology. 2020 Dec 9;3(2):000185. DOI:10.1099/acmi.0.000185 2020
The prevalence of pilus islets in Streptococcus pneumoniae isolates from healthy children in Indonesia [0.03%]
印尼健康儿童中肺炎链球菌分离株的菌毛岛流行率
Dodi Safari,Feby Valentiya,Korrie Salsabila et al.
Dodi Safari et al.
Streptococcus pneumoniae produces pili that function as adherence factors to bind to epithelial cells in the human upper respiratory tract. In this study, we investigated the prevalence of pilus islets (PIs) in S. pneumoniae strains carried...