David Ross,Peter D Tonner,Olga B Vasilyeva
David Ross
Microbial cell culture is one of the most commonly performed protocols for synthetic biology, and laboratories are increasingly using 96-well plates and laboratory automation systems for cell culture. Here, we describe a method for reproduc...
Evaluating the persistence and stability of a DNA-barcoded microbial system in a mock home environment [0.03%]
评估模拟居家环境中DNA条形码微生物系统的持久性和稳定性
Nathan D McDonald,Katherine A Rhea,John P Davies Jr et al.
Nathan D McDonald et al.
Recent advancements in engineered microbial systems capable of deployment in complex environments have enabled the creation of unique signatures for environmental forensics operations. These microbial systems must be robust, able to thrive ...
Effects of DNA template preparation on variability in cell-free protein production [0.03%]
DNA模板制备对无细胞蛋白质合成变化性的影响研究
Eugenia Romantseva,Nina Alperovich,David Ross et al.
Eugenia Romantseva et al.
DNA templates for protein production remain an unexplored source of variability in the performance of cell-free expression (CFE) systems. To characterize this variability, we investigated the effects of two common DNA extraction methodologi...
The transcription activator AtxA from Bacillus anthracis was employed for developing a tight-control, high-level, modulable and stationary-phase-specific transcription activity in Escherichia coli [0.03%]
来自炭疽芽胞杆菌的转录激活因子AtxA在大肠杆菌中开发一种严密控制、高水平、可调节且只在稳定期特异性转录活性中的应用
Ying-Xing Liu,Xiao-Zhen Zhuo,Si-Yu Li
Ying-Xing Liu
The strong transcriptional activity of the virulent gene pagA in Bacillus anthracis has been proven to be anthrax toxin activator (AtxA)-regulated. However, the obscure pagA transcription mechanism hinders practical applications of this str...
A toolkit for enhanced reproducibility of RNASeq analysis for synthetic biologists [0.03%]
合成生物学家的RNA序列分析增强可重复性工具包
Benjamin J Garcia,Joshua Urrutia,George Zheng et al.
Benjamin J Garcia et al.
Sequencing technologies, in particular RNASeq, have become critical tools in the design, build, test and learn cycle of synthetic biology. They provide a better understanding of synthetic designs, and they help identify ways to improve and ...
Variability in cell-free expression reactions can impact qualitative genetic circuit characterization [0.03%]
无细胞表达反应的变异性会影响遗传线路的定性特征分析
Katherine A Rhea,Nathan D McDonald,Stephanie D Cole et al.
Katherine A Rhea et al.
Cell-free expression systems provide a suite of tools that are used in applications from sensing to biomanufacturing. One of these applications is genetic circuit prototyping, where the lack of cloning is required and a high degree of contr...
Jacob Beal,Cheryl A Telmer,Alejandro Vignoni et al.
Jacob Beal et al.
Plate readers are commonly used to measure cell growth and fluorescence, yet the utility and reproducibility of plate reader data is limited by the fact that it is typically reported in arbitrary or relative units. We have previously establ...
Signal amplification of araC pBAD using a standardized translation initiation region [0.03%]
采用标准化的翻译起始区域放大araC-pbad信号
Patrick J Shilling,Diana Khananisho,Alister J Cumming et al.
Patrick J Shilling et al.
araC pBAD is a genetic fragment that regulates the expression of the araBAD operon in bacteria, which is required for the metabolism of L-arabinose. It is widely used in bioengineering applications because it can drive regulatable and titra...
Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform [0.03%]
传统的protocols和优化方法导致支原体无细胞基因表达平台表达量偏低
Andrei Sakai,Christopher R Deich,Frank H T Nelissen et al.
Andrei Sakai et al.
Cell-free expression (CFE) systems are one of the main platforms for building synthetic cells. A major drawback is the orthogonality of cell-free systems across species. To generate a CFE system compatible with recently established minimal ...