High-efficiency retron-mediated single-stranded DNA production in plants [0.03%]
植物中高效反式作用元件介导的单链DNA产生
Wenjun Jiang,Gundra Sivakrishna Rao,Rashid Aman et al.
Wenjun Jiang et al.
Retrons are a class of retroelements that produce multicopy single-stranded DNA (ssDNA) and participate in anti-phage defenses in bacteria. Retrons have been harnessed for the overproduction of ssDNA, genome engineering and directed evoluti...
An arrayed CRISPR screen reveals Myc depletion to increase productivity of difficult-to-express complex antibodies in CHO cells [0.03%]
阵列CRISPR筛选显示Myc耗竭可提高CHO细胞中难以表达的复杂抗体的生产力
Niels Bauer,Benedikt Oswald,Maximilian Eiche et al.
Niels Bauer et al.
Complex therapeutic antibody formats, such as bispecifics (bsAbs) or cytokine fusions, may provide new treatment options in diverse disease areas. However, the manufacturing yield of these complex antibody formats in Chinese Hamster Ovary (...
An injectable CRISPR therapy instructs B cells to produce anti-HIV antibodies [0.03%]
可注射的CRISPR疗法指导B细胞生产抗HIV抗体
Logan Thrasher Collins
Logan Thrasher Collins
Hurdles in responsive community engagement for the development of environmental biotechnologies [0.03%]
环境生物技术发展中响应型社区参与的障碍
Avery M Normandin,Lily M Fitzgerald,Julianne Yip et al.
Avery M Normandin et al.
Recent calls for engaging communities in biotechnology development do not draw enough attention to the hurdles that must be overcome for engagement strategies to effectively feed back into research design and conduct. These hurdles call int...
basicsynbio and the BASIC SEVA collection: software and vectors for an established DNA assembly method [0.03%]
基础合成生物学和BASIC SEVA系列：适用于已建立的DNA组装方法的软件和载体
Matthew C Haines,Benedict Carling,James Marshall et al.
Matthew C Haines et al.
Standardized deoxyribonucleic acid (DNA) assembly methods utilizing modular components provide a powerful framework to explore designs and iterate through Design-Build-Test-Learn cycles. Biopart Assembly Standard for Idempotent Cloning (BAS...
David M Truong
David M Truong
Highly-automated, high-throughput replication of yeast-based logic circuit design assessments [0.03%]
一种高效的酵母基逻辑电路评估的自动化高通量复制方法
Robert P Goldman,Robert Moseley,Nicholas Roehner et al.
Robert P Goldman et al.
We describe an experimental campaign that replicated the performance assessment of logic gates engineered into cells of Saccharomyces cerevisiae by Gander et al. Our experimental campaign used a novel high-throughput experimentation framewo...
Accurate characterization of dynamic microbial gene expression and growth rate profiles [0.03%]
精确表征动态微生物基因表达及生长速率谱型
Gonzalo Vidal,Carlos Vidal-Céspedes,Macarena Muñoz Silva et al.
Gonzalo Vidal et al.
Genetic circuits are subject to variability due to cellular and compositional contexts. Cells face changing internal states and environments, the cellular context, to which they sense and respond by changing their gene expression and growth...
René Inckemann
René Inckemann
Rahmi Lale,Lisa Tietze,Maxime Fages-Lartaud et al.
Rahmi Lale et al.
In this study, we provide a universal approach to Gene Expression Engineering (GeneEE) for creating artificial expression systems. GeneEE leads to the generation of artificial 5' regulatory sequences (ARES) consisting of promoters and 5' un...