Variability in genome-engineering source materials: consider your starting point [0.03%]
基因组工程起点材料的变异性：考虑您的起点
Simona Patange,Sierra D Miller,Samantha D Maragh
Simona Patange
The presence and impact of variability in cells as the source material for genome engineering are important to consider for the design, execution and interpretation of outcomes of a genome-engineering process. Variability may be present at ...
Akiko Kashiwagi,Tetsuya Yomo
Akiko Kashiwagi
How the ribonucleic acid (RNA) world transited to the deoxyribonucleic acid (DNA) world has remained controversial in evolutionary biology. At a certain time point in the transition from the RNA world to the DNA world, 'RNA replicons', in w...
Michael C Robitaille,Jeff M Byers,Joseph A Christodoulides et al.
Michael C Robitaille et al.
Live-cell imaging is extremely common in synthetic biology research, but its ability to be applied reproducibly across laboratories can be hindered by a lack of standardized image analysis. Here, we introduce a novel cell segmentation metho...
AssemblyTron: flexible automation of DNA assembly with Opentrons OT-2 lab robots [0.03%]
AssemblyTron：使用Opentrons OT-2实验室机器人灵活地实现DNA组装自动化
John A Bryant Jr,Mason Kellinger,Cameron Longmire et al.
John A Bryant Jr et al.
As one of the newest fields of engineering, synthetic biology relies upon a trial-and-error Design-Build-Test-Learn (DBTL) approach to simultaneously learn how a function is encoded in biology and attempt to engineer it. Many software and h...
Ana Delgado
Ana Delgado
Reusability has been a key issue since the origins of the parts-based approach to synthetic biology. Starting with the BioBrick™ standard part, multiple efforts have aimed to make biology more exchangeable. The reusability of parts and oth...
Development of an expression-tunable multiple protein synthesis system in cell-free reactions using T7-promoter-variant series [0.03%]
使用T7启动子变异系列在无细胞反应中开发表达可调的多蛋白合成系统的发展
Naoko Senda,Toshihiko Enomoto,Kenta Kihara et al.
Naoko Senda et al.
New materials with a low environmental load are expected to be generated through synthetic biology. To widely utilize this technology, it is important to create cells with designed biological functions and to control the expression of multi...
Editing Aspergillus terreus using the CRISPR-Cas9 system [0.03%]
利用CRISPR-Cas9系统改造Aspergillus terreus
Sra-Yh Shih,Uffe Hasbro Mortensen,Fang-Rong Chang et al.
Sra-Yh Shih et al.
CRISPR-Cas9 technology has been utilized in different organisms for targeted mutagenesis, offering a fast, precise and cheap approach to speed up molecular breeding and study of gene function. Until now, many researchers have established th...