Advancing reproducibility can ease the 'hard truths' of synthetic biology [0.03%]
推进合成生物学的可重复性或能缓解其“艰难真相”问题
Matthew W Lux,Elizabeth A Strychalski,Gary J Vora
Matthew W Lux
Reproducibility has been identified as an outstanding challenge in science, and the field of synthetic biology is no exception. Meeting this challenge is critical to allow the transformative technological capabilities emerging from this fie...
Rewiring cell-free metabolic flux in E. coli lysates using a block-push-pull approach [0.03%]
使用模块化阻断-推动-拉动方法重构大肠杆菌胞浆内的代谢路径
Jaime Lorenzo N Dinglasan,Mitchel J Doktycz
Jaime Lorenzo N Dinglasan
Cell-free systems can expedite the design and implementation of biomanufacturing processes by bypassing troublesome requirements associated with the use of live cells. In particular, the lack of survival objectives and the open nature of ce...
Periplasmic stress contributes to a trade-off between protein secretion and cell growth in Escherichia coli Nissle 1917 [0.03%]
周质压力导致乳杆菌Nissle 1917蛋白分泌和细胞生长间的权衡关系
Sivaram Subaya Emani,Anton Kan,Timothy Storms et al.
Sivaram Subaya Emani et al.
Maximizing protein secretion is an important target in the design of engineered living systems. In this paper, we characterize a trade-off between cell growth and per-cell protein secretion in the curli biofilm secretion system of Escherich...
pBLAM1-x: standardized transposon tools for high-throughput screening [0.03%]
pBLAM1-x：用于高通量筛选的转座子工具标准化
Lorea Alejaldre,Ana-Mariya Anhel,Ángel Goñi-Moreno
Lorea Alejaldre
The engineering of pre-defined functions in living cells requires increasingly accurate tools as synthetic biology efforts become more ambitious. Moreover, the characterization of the phenotypic performance of genetic constructs demands met...
Biosynthesis of cannabigerol and cannabigerolic acid: the gateways to further cannabinoid production [0.03%]
大麻二酚和大麻二酚酸的生物合成：进一步生成大麻素的途径
Lewis J Kearsey,Cunyu Yan,Nicole Prandi et al.
Lewis J Kearsey et al.
Cannabinoids are a therapeutically valuable class of secondary metabolites with a vast number of substituents. The native cannabinoid biosynthetic pathway of Cannabis sativa generates cannabigerolic acid (CBGA), the common substrate to mult...
Fighting fire with fire: engineering a microbe into a therapeutic defense against drug-resistant biofilms [0.03%]
以火攻火：工程改造微生物成为对抗耐药生物膜的治疗防御手段
Charlotte Ayn Cialek
Charlotte Ayn Cialek
Lalitha S Sundaram,James W Ajioka,Jennifer C Molloy
Lalitha S Sundaram
While synthetic biology is hoped to hold promise and potential to address pressing global challenges, the issue of regulation is an under-appreciated challenge. Particularly in Europe, the regulatory frameworks involved are rooted in histor...
Functional Synthetic Biology [0.03%]
功能合成生物学
Ibrahim Aldulijan,Jacob Beal,Sonja Billerbeck et al.
Ibrahim Aldulijan et al.
Synthetic biologists have made great progress over the past decade in developing methods for modular assembly of genetic sequences and in engineering biological systems with a wide variety of functions in various contexts and organisms. How...
Robustness and reproducibility of simple and complex synthetic logic circuit designs using a DBTL loop [0.03%]
基于DBTL循环的合成逻辑电路设计的稳健性和可重复性研究——从简单到复杂
Breschine Cummins,Justin Vrana,Robert C Moseley et al.
Breschine Cummins et al.
Computational tools addressing various components of design-build-test-learn (DBTL) loops for the construction of synthetic genetic networks exist but do not generally cover the entire DBTL loop. This manuscript introduces an end-to-end seq...