Ludo L J Schoenmakers,Max J den Uijl,Jelle L Postma et al.
Ludo L J Schoenmakers et al.
Giant unilamellar vesicles (GUVs) provide a powerful model compartment for synthetic cells. However, a key challenge is the incorporation of membrane proteins that allow for transport, energy transduction, compartment growth and division. H...
Sonja Billerbeck
Sonja Billerbeck
Screening putative polyester polyurethane degrading enzymes with semi-automated cell-free expression and nitrophenyl probes [0.03%]
半自动化无细胞表达和硝基苯探针筛选疑似聚酯聚氨酯降解酶
Afrin Ahsan,Dominique Wagner,Vanessa A Varaljay et al.
Afrin Ahsan et al.
Cell-free expression (CFE) has shown recent utility in prototyping enzymes for discovery efforts. In this work, CFE is demonstrated as an effective tool to screen putative polyester polyurethane degrading enzyme sequences sourced from metag...
Preparing for the future of precision medicine: synthetic cell drug regulation [0.03%]
为精准医疗的未来做准备：合成细胞药物监管
Kira Sampson,Carlise Sorenson,Katarzyna P Adamala
Kira Sampson
Synthetic cells are a novel class of cell-like bioreactors, offering the potential for unique advancements in synthetic biology and biomedicine. To realize the potential of those technologies, synthetic cell-based drugs need to go through t...
On the path to generate electricity from wastewater through genetic engineering of Escherichia coli [0.03%]
通过基因工程大肠杆菌产生污水电力之路
Charlotte Ayn Cialek
Charlotte Ayn Cialek
The naringenin-dependent regulator FdeR can be applied as a NIMPLY gate controlled by naringenin and arabinose [0.03%]
那林醇依赖性调节因子FdeR可作为由那林醇和阿拉伯糖调控的NIMPLY门电路
Fernanda Miyuki Kashiwagi,Brenno Wendler Miranda,Emanuel Maltempi de Souza et al.
Fernanda Miyuki Kashiwagi et al.
The FdeR regulator has been reported as a transcriptional activator dependent on the interaction with naringenin. Previously, FdeR and its cognate promoter were used to construct naringenin-sensitive sensors, though no correlation was assoc...
Cell-free expression of RuBisCO for ATP production in the synthetic cells [0.03%]
无细胞表达核酮糖-5磷酸羧化酶/还原酶以在人工细胞中产生ATP
Shugo Sugii,Katsumi Hagino,Ryo Mizuuchi et al.
Shugo Sugii et al.
Recent advances in bottom-up synthetic biology have made it possible to reconstitute cellular systems from non-living components, yielding artificial cells with potential applications in industry, medicine and basic research. Although a var...
Utilizing a cell-free protein synthesis platform for the biosynthesis of a natural product, caffeine [0.03%]
利用无细胞蛋白质合成平台进行天然产物——咖啡因的生物合成
Alexander Ditzel,Fanglong Zhao,Xue Gao et al.
Alexander Ditzel et al.
Natural products are a valuable source of pharmaceuticals, providing a majority of the small-molecule drugs in use today. However, their production through organic synthesis or in heterologous hosts can be difficult and time-consuming. Ther...
Matthew W Lux,Elizabeth A Strychalski,Gary J Vora
Matthew W Lux