OT-Mation: an open-source code for parsing CSV files into Python scripts for control of OT-2 liquid-handling robotics [0.03%]
OT-Mation：一种开源代码 将CSV文件解析到Python脚本中 以控制OT-2液操机器人
Alex Laverick,Katherine Convey,Catherine Harrison et al.
Alex Laverick et al.
OT-Mation is an open-source Python script designed to automate the programming of OT-2 liquid-handling robots, making combinatorial experiments more accessible to researchers. By parsing user-defined CSV files containing information on labw...
Engineering a custom-sized DNA scaffold for more efficient DNA origami-based nucleic acid data storage [0.03%]
工程化定制大小的DNA支架以更高效地进行基于DNA折纸技术的核酸数据存储
Sarah E Kobernat,Maryna Lazouskaya,Benjamin C Balzer et al.
Sarah E Kobernat et al.
DNA has emerged as a promising material to address growing data storage demands. We recently demonstrated a structure-based DNA data storage approach where DNA probes are spatially oriented on the surface of DNA origami and decoded using DN...
Two highly specific growth-coupled biosensor for glycolaldehyde detection across micromolar and millimolar concentrations [0.03%]
两个与生长耦合的高度特异性生物传感器用于微摩尔和毫摩尔浓度下甘油醛的检测
Paul A Gómez-Coronado,Armin Kubis,Maria Kowald et al.
Paul A Gómez-Coronado et al.
Glycolaldehyde (GA), the smallest sugar, has significant potential as a biomass-derived platform chemical and is a key metabolite in several synthetic pathways for one-carbon metabolism and new-to-nature photorespiration. This study introdu...
In silico prediction of loop-mediated isothermal amplification using a generalized linear model [0.03%]
基于广义线性模型的环介导等温扩增的计算机预测方法研究
Kenshiro Taguchi,Satoru Michiyuki,Takumasa Tsuji et al.
Kenshiro Taguchi et al.
Loop-mediated isothermal amplification (LAMP), a DNA amplification technique under isothermal conditions, provides the important benefits of high sensitivity, specificity, rapidity, and simplicity. Maximizing LAMP features necessitates the ...
Simulations predict stronger CRISPRi transcriptional repression in plants for identical than heterogeneous gRNA target sites [0.03%]
模拟预测对于相同的gRNA目标位点，植物CRISPRi转录阻遏比异质性更强
Helen Scott,Alessandro Occhialini,Scott C Lenaghan et al.
Helen Scott et al.
Plant synthetic biologists have been working to adapt the CRISPRa and CRISPRi promoter regulation methods for applications such as improving crops or installing other valuable pathways. With other organisms, strong transcriptional control h...
Cell-free biosensor with automated acoustic liquid handling for rapid and scalable characterization of cellobiohydrolases on microcrystalline cellulose [0.03%]
一种基于声控液滴处理的无细胞生物传感器及其快速表征微晶纤维素上 cellobiohydrolases 的应用
Taeok Kim,Eun Jung Jeon,Kil Koang Kwon et al.
Taeok Kim et al.
Engineering enzymes to degrade solid substrates, such as crystalline cellulose from paper sludge or microplastics in sewage sludge, presents challenges for high-throughput screening (HTS), as solid substrates are not readily accessible in c...
biGMamAct: efficient CRISPR/Cas9-mediated docking of large functional DNA cargoes at the ACTB locus [0.03%]
biGMamAct：CRISPR / Cas9介导的大功能DNA载体在ACTB基因座的有效对接
Martin Pelosse,Marco Marcia
Martin Pelosse
Recent advances in molecular and cell biology and imaging have unprecedentedly enabled multiscale structure-functional studies of entire metabolic pathways from atomic to micrometer resolution and the visualization of macromolecular complex...
Biosensor characterization: formal methods from the perspective of proteome fractions [0.03%]
生物传感器特性分析：从蛋白质组分的角度看正式方法
Nicolás A Vaccari,Dahlin Zevallos-Aliaga,Tom Peeters et al.
Nicolás A Vaccari et al.
Many studies characterize transcription factors and other regulatory elements to control gene expression in recombinant systems. However, most lack a formal approach to analyse the inherent and context-specific variations of these regulator...
Andrew A Mishin,Tobin Groth,Richard E Green et al.
Andrew A Mishin et al.
In this study, we introduce a new in vitro method for oligonucleotide fragment assembly. Unlike polymerase chain assembly and ligase chain assembly that rely on short, highly purified oligonucleotides, our method, named Splynthesis, uses a ...
CryptKeeper: a negative design tool for reducing unintentional gene expression in bacteria [0.03%]
密码守护者：减少细菌中非故意基因表达的负向设计工具
Cameron T Roots,Jeffrey E Barrick
Cameron T Roots
Foundational techniques in molecular biology-such as cloning genes, tagging biomolecules for purification or identification, and overexpressing recombinant proteins-rely on introducing non-native or synthetic DNA sequences into organisms. T...