Positive-feedback, ratiometric biosensor expression improves high-throughput metabolite-producer screening efficiency in yeast [0.03%]
正反馈，比例生物传感器表达提高酵母菌高通量代谢产物生产者筛选效率
Thomas C Williams,Xin Xu,Martin Ostrowski et al.
Thomas C Williams et al.
Biosensors are valuable and versatile tools in synthetic biology that are used to modulate gene expression in response to a wide range of stimuli. Ligand responsive transcription factors are a class of biosensor that can be used to couple i...
Jean Peccoud
Jean Peccoud
Since the description, in 2000, of two artificial gene networks, synthetic biology has emerged as a new engineering discipline that catalyzes a change of culture in the life sciences. Recombinant DNA can now be fabricated rather than cloned...
Namrita Dhillon,Robert Shelansky,Brent Townshend et al.
Namrita Dhillon et al.
Gene expression in Saccharomyces cerevisiae is regulated at multiple levels. Genomic and epigenomic mapping of transcription factors and chromatin factors has led to the delineation of various modular regulatory elements-enhancers (upstream...
Universal loop assembly: open, efficient and cross-kingdom DNA fabrication [0.03%]
通用型环状装配技术：开放、高效和跨物种DNA制造技术
Bernardo Pollak,Tamara Matute,Isaac Nuñez et al.
Bernardo Pollak et al.
Standardized type IIS DNA assembly methods are becoming essential for biological engineering and research. These methods are becoming widespread and more accessible due to the proposition of a 'common syntax' that enables higher interoperab...
Better research by efficient sharing: evaluation of free management platforms for synthetic biology designs [0.03%]
高效分享下的更好研究——合成生物学设计免费管理平台的评估
Uriel Urquiza-García,Tomasz Zieliński,Andrew J Millar
Uriel Urquiza-García
Synthetic biology aims to introduce engineering principles into biology, for example, the construction of biological devices by assembling previously-characterized, functional parts. This approach demands new resources for cataloging and sh...
Brian E Hew,Ryuei Sato,Damiano Mauro et al.
Brian E Hew et al.
Safer and more efficient methods for directing therapeutic genes to specific sequences could increase the repertoire of treatable conditions. Many current approaches act passively, first initiating a double-stranded break, then relying on h...
CRISPR-interference-based modulation of mobile genetic elements in bacteria [0.03%]
基于CRISPR干扰的细菌可移动遗传元件调控
Ákos Nyerges,Balázs Bálint,Judit Cseklye et al.
Ákos Nyerges et al.
Spontaneous mutagenesis of synthetic genetic constructs by mobile genetic elements frequently results in the rapid loss of engineered functions. Previous efforts to minimize such mutations required the exceedingly time-consuming manipulatio...
Construction of a novel phagemid to produce custom DNA origami scaffolds [0.03%]
构建新型噬菌体衍生质粒以生产自定义DNA折纸长支架骨架序列
Parsa M Nafisi,Tural Aksel,Shawn M Douglas
Parsa M Nafisi
DNA origami, a method for constructing nanoscale objects, relies on a long single strand of DNA to act as the 'scaffold' to template assembly of numerous short DNA oligonucleotide 'staples'. The ability to generate custom scaffold sequences...
Cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates [0.03%]
基于酶强化大肠杆菌裂解物的细胞 free 柠檬烯生物合成
Quentin M Dudley,Connor J Nash,Michael C Jewett
Quentin M Dudley
Isoprenoids are an attractive class of metabolites for enzymatic synthesis from renewable substrates. However, metabolic engineering of microorganisms for monoterpenoid production is limited by the need for time-consuming, and often non-int...
Low-cost detection of norovirus using paper-based cell-free systems and synbody-based viral enrichment [0.03%]
基于合成抗体的病毒富集在纸基无细胞系统中检测诺如病毒的新方法
Duo Ma,Luhui Shen,Kaiyue Wu et al.
Duo Ma et al.
Noroviruses are a primary cause of gastroenteritis and foodborne illness with cases that affect millions of people worldwide each year. Inexpensive tests for norovirus that do not require sophisticated laboratory equipment are important too...