Cell-free TXTL synthesis of infectious bacteriophage T4 in a single test tube reaction [0.03%]
单试管反应中无细胞TXTL合成具有感染性的T4噬菌体
Mark Rustad,Allen Eastlund,Paul Jardine et al.
Mark Rustad et al.
The bottom-up construction of biological entities from genetic information provides a broad range of opportunities to better understand fundamental processes within living cells, as well as holding great promise for the development of novel...
Engineering DNA nanotubes for resilience in an E. coli TXTL system [0.03%]
为了在大肠杆菌TXTL系统中增强韧性而对DNA纳米管进行工程化设计
Melissa A Klocke,Jonathan Garamella,Hari K K Subramanian et al.
Melissa A Klocke et al.
Deoxyribonucleic acid (DNA) nanotechnology is a growing field with potential intracellular applications. In this work, we use an Escherichia coli cell-free transcription-translation (TXTL) system to assay the robustness of DNA nanotubes in ...
A set of experimentally validated, mutually orthogonal primers for combinatorially specifying genetic components [0.03%]
一组经实验验证的相互正交的引物，用于组合指定遗传元件
Subu K Subramanian,William P Russ,Rama Ranganathan
Subu K Subramanian
The design and synthesis of novel genes and deoxyribonucleic acid (DNA) sequences is a central technique in synthetic biology. Current methods of high throughput gene synthesis use pooled oligonucleotides obtained from custom-designed DNA m...
Tailor-made exopolysaccharides-CRISPR-Cas9 mediated genome editing in Paenibacillus polymyxa [0.03%]
CRISPR-Cas9介导的Paenibacillus polymyxa基因编辑-为特制的外源多糖所做的努力
Marius Rütering,Brady F Cress,Martin Schilling et al.
Marius Rütering et al.
Application of state-of-the-art genome editing tools like CRISPR-Cas9 drastically increase the number of undomesticated micro-organisms amenable to highly efficient and rapid genetic engineering. Adaptation of these tools to new bacterial f...
Design and evaluation of an incoherent feed-forward loop for an arsenic biosensor based on standard iGEM parts [0.03%]
基于标准iGEM零件的砷生物传感器的非相干前馈环路的设计与评估
Federico Barone,Francisco Dorr,Luciano E Marasco et al.
Federico Barone et al.
The diversity and flexibility of life offers a wide variety of molecules and systems useful for biosensing. A biosensor device should be robust, specific and reliable. Inorganic arsenic is a highly toxic water contaminant with worldwide dis...
Translation inhibition and resource balance in the TX-TL cell-free gene expression system [0.03%]
翻译抑制与资源平衡在TX-TL无细胞基因表达系统中的作用
Vijayalakshmi H Nagaraj,James M Greene,Anirvan M Sengupta et al.
Vijayalakshmi H Nagaraj et al.
Quantifying the effect of vital resources on transcription (TX) and translation (TL) helps to understand the degree to which the concentration of each resource must be regulated for achieving homeostasis. Utilizing the synthetic TX-TL syste...
Exploiting the sequence diversity of TALE-like repeats to vary the strength of dTALE-promoter interactions [0.03%]
利用类似TALE的重复序列多样性来改变dTALE-启动子相互作用的强度
Orlando de Lange,Niklas Schandry,Markus Wunderlich et al.
Orlando de Lange et al.
Designer transcription activator-like effectors (dTALEs) are programmable transcription factors used to regulate user-defined promoters. The TALE DNA-binding domain is a tandem series of amino acid repeats that each bind one DNA base. Each ...
Ken-Ichi Yamazaki,Kim de Mora,Kensuke Saitoh
Ken-Ichi Yamazaki
Because of the technological limitations of de novo DNA synthesis in (i) making constructs containing tandemly repeated DNA sequence units, (ii) making an unbiased DNA library containing DNA fragments with sequence multiplicity in a specifi...
Chun Tung Chou
Chun Tung Chou
Living cells constantly process information from their living environment. It has recently been shown that a number of cell signaling mechanisms (e.g. G protein-coupled receptor and epidermal growth factor) can be interpreted as computing t...
ACRE: Absolute concentration robustness exploration in module-based combinatorial networks [0.03%]
ACRE：基于模块组合网络的绝对浓度稳健性探索
Hiroyuki Kuwahara,Ramzan Umarov,Islam Almasri et al.
Hiroyuki Kuwahara et al.
To engineer cells for industrial-scale application, a deep understanding of how to design molecular control mechanisms to tightly maintain functional stability under various fluctuations is crucial. Absolute concentration robustness (ACR) i...