Expanding the Yeast MoClo Toolkit: gene expression control parts for Saccharomyces cerevisiae tested in industrially relevant conditions [0.03%]
用于酿酒酵母的基因表达调控元件的工业条件下的筛选及酵母MoClo工具组的扩展
Elin Blick,Yvonne Nygård
Elin Blick
Fine-tuning of gene expression is often required to achieve competitive production levels in microbial cell factories. Several orthogonal expression systems based on heterologous regulatory parts have been developed for Saccharomyces cerevi...
Engineering artificial 5' regulatory sequences for thermostable protein expression in the extremophile Thermus thermophilus [0.03%]
工程极端耐热菌 Thermus thermophilus 表达系统中热稳定性蛋白质的5'调控序列研究
Che Fai Alex Wong,Shizhe Zhang,Lisa Tietze et al.
Che Fai Alex Wong et al.
The utilization of biocatalysts in biotechnological applications often necessitates their heterologous expression in suitable host organisms. However, the range of standardized microbial hosts for recombinant protein production remains limi...
GoldenBraid2.0 E. coli: a comprehensive and characterized toolkit for enterics [0.03%]
金式绳结2.0大肠杆菌版本：一套全面且经表征的构建模块用于沙门氏菌合成生物学研究
Matthew B Cooke,Kobie T Welch,Laura D Ramirez et al.
Matthew B Cooke et al.
Modular cloning systems streamline laboratory workflows by consolidating genetic 'parts' into reusable and modular collections, enabling researchers to fast-track strain construction. The GoldenBraid 2.0 modular cloning system utilizes the ...
A mathematical model of cell-free transcription-translation with plasmid crosstalk [0.03%]
一种关于质粒串扰的无细胞转录翻译数学模型
Yue Han,Alexandra T Patterson,Fernanda Piorino et al.
Yue Han et al.
Cell-free expression (CFE) systems are emerging as a powerful tool in synthetic biology, with diverse applications from prototyping genetic circuits to serving as a platform for point-of-care biosensors. When multiple genes need to be expre...
Charlotte Ayn Cialek
Charlotte Ayn Cialek
Construction of a syntrophic Pseudomonas putida consortium with reciprocal substrate processing [0.03%]
一种相互依存的Pseudomonas putida菌群的构建及互为底物的处理方法
Barbora Burýšková,Jesús Miró-Bueno,Barbora Popelářová et al.
Barbora Burýšková et al.
Synthetic microbial consortia can leverage their expanded enzymatic reach to tackle biotechnological challenges too complex for single strains, such as biosynthesis of complex secondary metabolites or waste plant biomass degradation and val...
A Golden Gate compatible system for continuous directed evolution in E. coli [0.03%]
一种兼容Golden Gate的系统 可用于大肠杆菌中的连续定向进化
Ignacio Sparrow Muñoz,Steven J Burgess
Ignacio Sparrow Muñoz
Directed evolution is a technique that allows for protein engineering without prior knowledge. Continuous directed evolution employs gene-specific hypermutation tied to functional selection within a single cell, enabling a broad search of s...
Lorenzo Scutteri,Patrick Barth,Sahand Jamal Rahi
Lorenzo Scutteri
Sophisticated genetic engineering tasks such as protein domain grafting and multi-gene fusions are hampered by the lack of suitable vector backbones. In particular, many restriction sites are in the backbone outside the polylinker region (m...
Oligonucleotide library assisted sequence mining reveals promoter sequences with distinct temporal expression dynamics for applications in Curvibacter sp. AEP1-3 [0.03%]
寡核苷酸文库辅助序列挖掘揭示了具有不同时间表达动态的启动子序列在Curvibacter sp. AEP1-3中的应用
Maurice Mager,Lukas Becker,Nina Schulten et al.
Maurice Mager et al.
The β-proteobacterial species Curvibacter sp. AEP1-3 is a model organism for the study of symbiotic interactions as it is the most abundant colonizer of Hydra vulgaris. Yet, genetic tools for Curvibacter are still in their infancy; few pro...
Synthetic biology design principles enable efficient bioproduction of Heparosan with low molecular weight and low polydispersion index for the biomedical industry [0.03%]
合成生物学设计原理可高效生产低分子量和低多分散指数的赫帕索san用于生物医药行业
Yadira Boada,Marcelo Flores,Martin Stiebritz et al.
Yadira Boada et al.
Heparosan is a natural polymer with unique chemical and biological properties, that holds great promise for biomedical applications. The molecular weight (Mw) and polydispersion index (PDI) are critical factors influencing the performance o...