rClone Red facilitates bacterial gene expression research by undergraduates in the teaching laboratory [0.03%]
rClone Red通过促进细菌基因表达研究方便了大学生在教学实验室的学习
A Malcolm Campbell,Todd T Eckdahl
A Malcolm Campbell
rClone Red is a low-cost and student-friendly research tool that has been used successfully in undergraduate teaching laboratories. It enables students to perform original research within the financial and time constraints of a typical unde...
High-throughput screening of biomolecules using cell-free gene expression systems [0.03%]
使用无细胞基因表达系统进行生物分子的高通量筛选
Luis E Contreras-Llano,Cheemeng Tan
Luis E Contreras-Llano
The incorporation of cell-free transcription and translation systems into high-throughput screening applications enables the in situ and on-demand expression of peptides and proteins. Coupled with modern microfluidic technology, the cell-fr...
Extrinsic stochastic factors (solute partition) in gene expression inside lipid vesicles and lipid-stabilized water-in-oil droplets: a review [0.03%]
脂质小泡和脂质稳定的水包油液滴内基因表达的外在随机因素（溶质分配）：综述
Emiliano Altamura,Paolo Carrara,Francesca D&#x;Angelo et al.
Emiliano Altamura et al.
The encapsulation of transcription-translation (TX-TL) machinery inside lipid vesicles and water-in-oil droplets leads to the construction of cytomimetic systems (often called 'synthetic cells') for synthetic biology and origins-of-life res...
Hybrid semiparametric systems for quantitative sequence-activity modeling of synthetic biological parts [0.03%]
合成生物部件定量序列活性建模的混合半参数系统
Rui M C Portela,Moritz von Stosch,Rui Oliveira
Rui M C Portela
Predicting the activity of modified biological parts is difficult due to the typically large size of nucleotide sequences, resulting in combinatorial designs that suffer from the "curse of dimensionality" problem. Mechanistic design methods...
Rational engineering of photosynthetic electron flux enhances light-powered cytochrome P450 activity [0.03%]
合理改造光合作用的电子流可增强光驱动细胞色素P450活性
Adokiye Berepiki,John R Gittins,C Mark Moore et al.
Adokiye Berepiki et al.
In this study, we exploited a modified photosynthetic electron transfer chain (PET) in the model cyanobacterium Synechococcus PCC 7002, where electrons derived from water-splitting are used to power reactions catalyzed by a heterologous cyt...
Takayuki Katoh,Toby Passioura,Hiroaki Suga
Takayuki Katoh
To date, various genetic code manipulation methods have been developed to introduce non-proteinogenic amino acids into peptides by translation. However, the number of amino acids that can be used simultaneously remains limited even using th...
Different protein localizations on the inner and outer leaflet of cell-sized liposomes using cell-free protein synthesis [0.03%]
使用细胞自由蛋白质合成在细胞大小脂质体的内层和外层定位不同的蛋白
Atsuko Uyeda,Takayoshi Watanabe,Takahiro Hohsaka et al.
Atsuko Uyeda et al.
Membranes of living cells possess asymmetry. The inner and outer leaflets of the membrane consist of different phospholipid compositions, which are known to affect the function of membrane proteins, and the loss of the asymmetry has been re...
Elucidating the potential of crude cell extracts for producing pyruvate from glucose [0.03%]
粗细胞提取物从葡萄糖生产丙酮酸的潜力分析
David C Garcia,Benjamin P Mohr,Jakob T Dovgan et al.
David C Garcia et al.
Living systems possess a rich biochemistry that can be harnessed through metabolic engineering to produce valuable therapeutics, fuels and fine chemicals. In spite of the tools created for this purpose, many organisms tend to be recalcitran...
Rapid production and characterization of antimicrobial colicins using Escherichia coli-based cell-free protein synthesis [0.03%]
利用大肠杆菌无细胞蛋白质合成系统快速生产及鉴定抗菌蛋白菌素
Xing Jin,Weston Kightlinger,Yong-Chan Kwon et al.
Xing Jin et al.
Colicins are antimicrobial proteins produced by Escherichia coli, which, upon secretion from the host, kill non-host E. coli strains by forming pores in the inner membrane and degrading internal cellular components such as DNA and RNA. Due ...
Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements [0.03%]
用于快速筛选基因调控元件的Pseudomonas putida无细胞蛋白质合成平台的开发
He Wang,Jian Li,Michael C Jewett
He Wang
Cell-free protein synthesis (CFPS) systems enable the production of protein without the use of living, intact cells. An emerging area of interest is to use CFPS systems to characterize individual elements for genetic programs [e.g. promoter...