Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes [0.03%]
扩展具有鸟嘌呤依赖性适配酶的合成核糖开关工具箱
Julia Stifel,Maike Spöring,Jörg Steffen Hartig
Julia Stifel
Artificial riboswitches based on ribozymes serve as versatile tools for ligand-dependent gene expression regulation. Advantages of these so-called aptazymes are their modular architecture and the comparably little coding space they require....
Konstantinos Vavitsas
Konstantinos Vavitsas
Benjamin A Blount,Tom Ellis
Benjamin A Blount
The Synthetic Genome Summer Course was convened with the aim of teaching a wide range of researchers the theory and practical skills behind recent advances in synthetic biology and synthetic genome science, with a focus on Sc2.0, the synthe...
Design of RNA hairpin modules that predictably tune translation in yeast [0.03%]
可预测地调节酵母翻译的RNA发夹模块的设计
Tim Weenink,Jelle van der Hilst,Robert M McKiernan et al.
Tim Weenink et al.
Modular parts for tuning translation are prevalent in prokaryotic synthetic biology but lacking for eukaryotic synthetic biology. Working in Saccharomyces cerevisiae yeast, we here describe how hairpin RNA structures inserted into the 5' un...
Engineering a living biomaterial via bacterial surface capture of environmental molecules [0.03%]
通过细菌表面捕获环境分子来工程化活体生物材料
Felicia Y Scott,Keith C Heyde,MaryJoe K Rice et al.
Felicia Y Scott et al.
Synthetic biology holds significant potential in biomaterials science as synthetically engineered cells can produce new biomaterials, or alternately, can function as living components of new biomaterials. Here, we describe the creation of a...
Cell-free prototyping strategies for enhancing the sustainable production of polyhydroxyalkanoates bioplastics [0.03%]
用于提高聚羟基烷酸酯生物塑料可持续生产的无细胞原型策略
Richard Kelwick,Luca Ricci,Soo Mei Chee et al.
Richard Kelwick et al.
The polyhydroxyalkanoates (PHAs) are microbially-produced biopolymers that could potentially be used as sustainable alternatives to oil-derived plastics. However, PHAs are currently more expensive to produce than oil-derived plastics. There...
Expanding the toolbox for Synechocystis sp. PCC 6803: validation of replicative vectors and characterization of a novel set of promoters [0.03%]
扩展Synechocystis sp. PCC 6803的工具箱：验证复制向量和表征新型启动子集合
Eunice A Ferreira,Catarina C Pacheco,Filipe Pinto et al.
Eunice A Ferreira et al.
Cyanobacteria are promising 'low-cost' cell factories since they have minimal nutritional requirements, high metabolic plasticity and can use sunlight and CO2 as energy and carbon sources. The unicellular Synechocystis sp. PCC 6803, already...