Dual UTR-A novel 5' untranslated region design for synthetic biology applications [0.03%]
双UTR-A新型5'非翻译区设计及其在合成生物学中的应用
Simone Balzer Le,Ingerid Onsager,Jon Andreas Lorentzen et al.
Simone Balzer Le et al.
Bacterial 5' untranslated regions of mRNA (UTR) involve in a complex regulation of gene expression; however, the exact sequence features contributing to gene regulation are not yet fully understood. In this study, we report the design of a ...
Genomics-enabled analysis of specialized metabolism in bioenergy crops: current progress and challenges [0.03%]
生物能源作物中专门代谢的基因组学使能分析：当前进展与挑战
Kira Tiedge,Andrew Muchlinski,Philipp Zerbe
Kira Tiedge
Plants produce a staggering diversity of specialized small molecule metabolites that play vital roles in mediating environmental interactions and stress adaptation. This chemical diversity derives from dynamic biosynthetic pathway networks ...
Biosensor-based enzyme engineering approach applied to psicose biosynthesis [0.03%]
用于psicose生物合成的基于生物传感器的酶工程方法
Jeremy Armetta,Rose Berthome,Antonin Cros et al.
Jeremy Armetta et al.
Bioproduction of chemical compounds is of great interest for modern industries, as it reduces their production costs and ecological impact. With the use of synthetic biology, metabolic engineering and enzyme engineering tools, the yield of ...
Rapid generation of sequence-diverse terminator libraries and their parameterization using quantitative Term-Seq [0.03%]
序列多样终止子库的快速生成及其通过定量Term-Seq的参数化
Andrew J Hudson,Hans-Joachim Wieden
Andrew J Hudson
Synthetic biology and the rational design and construction of biological devices require vast numbers of characterized biological parts, as well as reliable design tools to build increasingly complex, multigene architectures. Design princip...
Highly multiplexed, fast and accurate nanopore sequencing for verification of synthetic DNA constructs and sequence libraries [0.03%]
用于验证合成DNA构建体和序列库的高复用、快速且准确的纳米孔测序技术
Andrew Currin,Neil Swainston,Mark S Dunstan et al.
Andrew Currin et al.
Synthetic biology utilizes the Design-Build-Test-Learn pipeline for the engineering of biological systems. Typically, this requires the construction of specifically designed, large and complex DNA assemblies. The availability of cheap DNA s...
Aaron J Dy,Emily R Aurand,Douglas C Friedman
Aaron J Dy
Online video resources have increasingly become a common way to effectively share scientific research ideas and engage viewers at many levels of interest or expertise. While synthetic biology is a comparatively young field, it has accumulat...
Niels Schlichting,Felix Reinhardt,Sven Jager et al.
Niels Schlichting et al.
The ligase cycling reaction (LCR) is a scarless and efficient method to assemble plasmids from fragments of DNA. This assembly method is based on the hybridization of DNA fragments with complementary oligonucleotides, so-called bridging oli...
Riboswitch identification using Ligase-Assisted Selection for the Enrichment of Responsive Ribozymes (LigASERR) [0.03%]
使用连接酶辅助选择来富集响应型核酶（LigASERR）识别核糖开关
Matthew C Haines,Marko Storch,Diego A Oyarzún et al.
Matthew C Haines et al.
In vitro selection of ligand-responsive ribozymes can identify rare, functional sequences from large libraries. While powerful, key caveats of this approach include lengthy and demanding experimental workflows; unpredictable experimental ou...