Sonja Billerbeck
Sonja Billerbeck
A biofoundry workflow for the identification of genetic determinants of microbial growth inhibition [0.03%]
微生物生长抑制的遗传决定因子的识别的合成生物学工作流程
Alaster D Moffat,Adam Elliston,Nicola J Patron et al.
Alaster D Moffat et al.
Biofoundries integrate high-throughput software and hardware platforms with synthetic biology approaches to enable the design, execution and analyses of large-scale experiments. The unique and powerful combination of laboratory infrastructu...
Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology [0.03%]
联合通用模块质粒（JUMP）：合成生物学的灵活载体平台
Marcos Valenzuela-Ortega,Christopher French
Marcos Valenzuela-Ortega
Generation of new DNA constructs is an essential process in modern life science and biotechnology. Modular cloning systems based on Golden Gate cloning, using Type IIS restriction endonucleases, allow assembly of complex multipart construct...
Michael J Hammerling,Danielle J Yoesep,Michael C Jewett
Michael J Hammerling
The ribosome is a two-subunit, macromolecular machine composed of RNA and proteins that carries out the polymerization of α-amino acids into polypeptides. Efforts to engineer ribosomal RNA (rRNA) deepen our understanding of molecular trans...
Directed evolution of synthetic coexistence: a new path towards ecosystem design? [0.03%]
合成共存的定向进化：生态系统设计的新路径？
Sonja Billerbeck
Sonja Billerbeck
Hailey I Edelstein,Patrick S Donahue,Joseph J Muldoon et al.
Hailey I Edelstein et al.
Synthetic receptors are powerful tools for engineering mammalian cell-based devices. These biosensors enable cell-based therapies to perform complex tasks such as regulating therapeutic gene expression in response to sensing physiological c...
CRAGE-mediated insertion of fluorescent chromosomal markers for accurate and scalable measurement of co-culture dynamics in Escherichia coli [0.03%]
CRAGE介导的荧光染色体标记插入技术在大肠杆菌共培养动力学研究中的精确和可扩展性测量中的应用
Avery J C Noonan,Yilin Qiu,Joe C H Ho et al.
Avery J C Noonan et al.
Monitoring population dynamics in co-culture is necessary in engineering microbial consortia involved in distributed metabolic processes or biosensing applications. However, it remains difficult to measure strain-specific growth dynamics in...
Advances in engineering CRISPR-Cas9 as a molecular Swiss Army knife [0.03%]
工程改造CRISPR-Cas9向分子瑞士军刀迈进
Grace A Meaker,Emma J Hair,Thomas E Gorochowski
Grace A Meaker
The RNA-guided endonuclease system CRISPR-Cas9 has been extensively modified since its discovery, allowing its capabilities to extend far beyond double-stranded cleavage to high fidelity insertions, deletions and single base edits. Such inn...
In silico design and automated learning to boost next-generation smart biomanufacturing [0.03%]
基于计算设计和自动学习的下一代智能制造工程学奠基性研究
Pablo Carbonell,Rosalind Le Feuvre,Eriko Takano et al.
Pablo Carbonell et al.
The increasing demand for bio-based compounds produced from waste or sustainable sources is driving biofoundries to deliver a new generation of prototyping biomanufacturing platforms. Integration and automation of the design, build, test an...
Modular cell-free expression plasmids to accelerate biological design in cells [0.03%]
一种用于细胞内生物设计的模块式无细胞表达质粒
Ashty S Karim,Fungmin Eric Liew,Shivani Garg et al.
Ashty S Karim et al.
Industrial biotechnology aims to produce high-value products from renewable resources. This can be challenging because model microorganisms-organisms that are easy to use like Escherichia coli-often lack the machinery required to utilize de...