Building a custom high-throughput platform at the Joint Genome Institute for DNA construct design and assembly-present and future challenges [0.03%]
联合基因组研究所DNA构建设计及组装的定制化高通量平台的建立及现在和未来面临的挑战
Ian K Blaby,Jan-Fang Cheng
Ian K Blaby
The rapid design and assembly of synthetic DNA constructs have become a crucial component of biological engineering projects via iterative design-build-test-learn cycles. In this perspective, we provide an overview of the workflows used to ...
Huseyin Tas,Lewis Grozinger,Angel Goñi-Moreno et al.
Huseyin Tas et al.
Boolean NOR gates have been widely implemented in Escherichia coli as transcriptional regulatory devices for building complex genetic circuits. Yet, their portability to other bacterial hosts/chassis is generally hampered by frequent change...
paraSBOLv: a foundation for standard-compliant genetic design visualization tools [0.03%]
paraSBOLv：用于符合标准的遗传设计可视化工具的基础
Charlie J Clark,James Scott-Brown,Thomas E Gorochowski
Charlie J Clark
Diagrams constructed from standardized glyphs are central to communicating complex design information in many engineering fields. For example, circuit diagrams are commonplace in electronics and allow for a suitable abstraction of the physi...
High-yield 'one-pot' biosynthesis of raspberry ketone, a high-value fine chemical [0.03%]
高产率的"一锅法"生物合成覆盆子酮——一种高价值精细化学品
Simon J Moore,Tommaso Tosi,David Bell et al.
Simon J Moore et al.
Cell-free extract and purified enzyme-based systems provide an attractive solution to study biosynthetic strategies towards a range of chemicals. 4-(4-hydroxyphenyl)-butan-2-one, also known as raspberry ketone, is the major fragrance compon...
Evaluation of inducible promoter-riboswitch constructs for heterologous protein expression in the cyanobacterial species Anabaena sp. PCC 7120 [0.03%]
用于念珠藻属外源蛋白表达的诱导型启动子-核糖开关结构的评价研究
Jessee Svoboda,Brenda Cisneros,Benjamin Philmus
Jessee Svoboda
Cyanobacteria are promising chassis for synthetic biology applications due to the fact that they are photosynthetic organisms capable of growing in simple, inexpensive media. Given their slower growth rate than other model organisms such as...
Methods for measuring the evolutionary stability of engineered genomes to improve their longevity [0.03%]
测量工程基因组的进化稳定性的方法以提高其持久性
Scott L Nuismer,Nathan C Layman,Alec J Redwood et al.
Scott L Nuismer et al.
Diverse applications rely on engineering microbes to carry and express foreign transgenes. This engineered baggage rarely benefits the microbe and is thus prone to rapid evolutionary loss when the microbe is propagated. For applications whe...
The all-E. coliTXTL toolbox 3.0: new capabilities of a cell-free synthetic biology platform [0.03%]
全大肠杆菌TXTL工具箱3.0：细胞外合成生物学平台的新功能
David Garenne,Seth Thompson,Amaury Brisson et al.
David Garenne et al.
The new generation of cell-free gene expression systems enables the prototyping and engineering of biological systems in vitro over a remarkable scope of applications and physical scales. As the utilization of DNA-directed in vitro protein ...
Binay Panda,Pawan K Dhar
Binay Panda
Biofoundry is a place where biomanufacturing meets automation. The highly modular structure of a biofoundry helps accelerate the design-build-test-learn workflow to deliver products fast and in a streamlined fashion. In this perspective, we...
David Bergenholm,Yasaman Dabirian,Raphael Ferreira et al.
David Bergenholm et al.
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has become a standard tool in many genome engineering endeavors. The endonuclease-deficient version of Cas9 (dCas9) is also a powerful programmable tool for ...
Tabasum Farzaneh,Paul S Freemont
Tabasum Farzaneh
Contemporary synthetic biology embraces the entire innovation pipeline; it is a transformative technology platform impacting new applications and improving existing industrial products and processes. However, challenges still emerge at the ...