Gur Pines,Assaf Pines,Carrie A Eckert
Gur Pines
Saturation mutagenesis is a semi-rational approach for protein engineering where sites are saturated either entirely or partially to include amino acids of interest. We previously reported on a codon compression algorithm, where a set of mi...
Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli [0.03%]
大肠杆菌中高效且可迭代的反转录介导的体内重组工程
Adam J Ellington,Christopher R Reisch
Adam J Ellington
Recombineering is an important tool in gene editing, enabling fast, precise and highly specific in vivo modification of microbial genomes. Oligonucleotide-mediated recombineering via the in vivo production of single-stranded DNA can overcom...
Xer recombination for the automatic deletion of selectable marker genes from plasmids in enteric bacteria [0.03%]
用于肠道细菌质粒中选择标记基因自动删除的Xer重组系统
Paola Salerno,Matthew W Leckenby,Bruce Humphrey et al.
Paola Salerno et al.
Antibiotic resistance genes are widely used to select bacteria transformed with plasmids and to prevent plasmid loss from cultures, yet antibiotics represent contaminants in the biopharmaceutical manufacturing process, and retaining antibio...
A linear programming-based strategy to save pipette tips in automated DNA assembly [0.03%]
基于线性规划的自动化DNA组装节省移液枪吸头策略
Kirill Sechkar,Zoltan A Tuza,Guy-Bart Stan
Kirill Sechkar
Laboratory automation and mathematical optimization are key to improving the efficiency of synthetic biology research. While there are algorithms optimizing the construct designs and synthesis strategies for DNA assembly, the optimization o...
SynBio2Easy-a biologist-friendly tool for batch operations on SBOL designs with Excel inputs [0.03%]
SynBio2Easy一个基于Excel的友好的SBOL设计批处理操作合成生物设计工具
Tomasz Zieliński,Johnny Hay,Andrew Romanowski et al.
Tomasz Zieliński et al.
Practical delivery of Findable, Accessible, Reusable and Interoperable principles for research data management requires expertise, time resource, (meta)data standards and formats, software tools and public repositories. The Synthetic Biolog...
Aoife M Brennan
Aoife M Brennan
Advances in synthetic biology have allowed the generation of strains of bacteria that are genetically altered to have specific therapeutic benefits. These synthetic biotics, also widely referred to as engineered living therapeutics, have tr...
Three overlooked key functional classes for building up minimal synthetic cells [0.03%]
构建最小合成细胞的三个被忽视的关键功能类群
Antoine Danchin
Antoine Danchin
Assembly of minimal genomes revealed many genes encoding unknown functions. Three overlooked functional categories account for some of them. Cells are prone to make errors and age. As a first key function, discrimination between proper and ...
Cofactor-independent RNA editing by a synthetic S-type PPR protein [0.03%]
一种合成的S型PPR蛋白独立于辅因子的RNA编辑作用
Kalia Bernath-Levin,Jason Schmidberger,Suvi Honkanen et al.
Kalia Bernath-Levin et al.
Pentatricopeptide repeat (PPR) proteins are RNA-binding proteins that are attractive tools for RNA processing in synthetic biology applications given their modular structure and ease of design. Several distinct types of motifs have been des...
GoldBricks: an improved cloning strategy that combines features of Golden Gate and BioBricks for better efficiency and usability [0.03%]
GoldBricks：结合Golden Gate和BioBricks特征以提高效率和可用性的改进型克隆策略
Vishalsingh R Chaudhari,Maureen R Hanson
Vishalsingh R Chaudhari
With increasing complexity of expression studies and the repertoire of characterized sequences, combinatorial cloning has become a common necessity. Techniques like BioBricks and Golden Gate aim to standardize and speed up the process of cl...
A CRISPR/Cas9 method facilitates efficient oligo-mediated gene editing in Debaryomyces hansenii [0.03%]
CRISPR / Cas9方法促进Debaryomyces hansenii中寡核苷酸介导的基因编辑efficient oligo-mediated基因编辑在Debaryomyces hansenii中高效地进行。
为了更符合中文表达习惯，可以进一步简化为：
CRISPR/Cas9技术促进德巴利马拉色菌中的寡核苷酸介导基因编辑
Tomas Strucko,Niklas L Andersen,Mikkel R Mahler et al.
Tomas Strucko et al.
Halophilic and osmotolerant yeast Debaryomyces hansenii has a high potential for cell factory applications due to its resistance to harsh environmental factors and compatibility with a wide substrate range. However, currently available gene...