A CURE for synthetic regulation of gene expression: rapid screening of guide RNA efficacy as a framework for enabling undergraduate research in plant synthetic biology [0.03%]
基于CRISPR的植物合成生物学本科生研究框架——快速筛选gRNA效率以治疗合成基因表达调控问题
Tawni Bull,Liz Carlsen,Noah Hoglund et al.
Tawni Bull et al.
Course-based Undergraduate Research Experiences (CUREs) have emerged as a transformative approach to science education, expanding access to authentic research opportunities beyond the traditional undergraduate research assistant (URA) train...
Chemically synthesized high-fidelity oligos ≤ 600 nt as building blocks to accelerate complex gene construction in synthetic biology [0.03%]
化学合成高保真性寡核苷酸（≤600nt）作为合成生物学中复杂基因构建的积木以加速复杂基因的构建
Mancang Zhang,Yang Hu,Hao Huang et al.
Mancang Zhang et al.
Synthetic biology and advanced genetic engineering applications rely heavily on the efficient construction of large and complex DNA sequences. Current DNA synthesis technologies have limited capacity to efficiently generate ultralong oligon...
Off-target detection of CRISPR-Cas9 nuclease in vitro with CROFT-Seq [0.03%]
基于CROFT-Seq的CRISPR-Cas9核酸酶脱靶检测
Paulius Toliusis,Algirdas Grybauskas,Tomas Sinkunas et al.
Paulius Toliusis et al.
Programmable CRISPR-Cas9 nucleases have become invaluable tools for genome editing. However, off-target cleavage by these nucleases can lead to unintended changes in the edited genome. Detection of off-target sites is critical to make genom...
Going beyond size: exploring the metabolic burden in Pseudomonas putida during heterologous protein production [0.03%]
超越大小：在恶臭假单胞菌中探索异源蛋白生产过程中的代谢负担
Marleen Beentjes,Ana-Sofia Ortega-Arbulú,Carina Meiners et al.
Marleen Beentjes et al.
In biotechnological applications, it is often necessary to introduce genes or entire pathways into a host cell, which can create a significant metabolic burden on the host, limiting productivity. In this study, we systematically investigate...
A protein-fragment complementation assay to quantify synthetic protein scaffold efficiency [0.03%]
一种量化合成蛋白质支架效率的蛋白质片段互补测定法
Pascale Lemieux,Alexandre K Dubé,Christian R Landry
Pascale Lemieux
Scaffolds are powerful tools in synthetic biology used for various applications, from increasing yield to optimizing signalling specificity. Protein scaffolds can be built by fusing peptide binding domains (PBD) and attaching the peptide th...
EASY-C: Extraction and Analysis of Small Yeast Chromosomes-A rapid and universal platform for recovering artificial mini-chromosomes from synthetic Sc2.0 yeast and large plasmids from Saccharomyces cerevisiae and nonconventional yeast species [0.03%]
从合成Sc2.0酵母和酿酒酵母及非传统酵母中快速分离人工小型染色体的通用平台EASY-C：小酵母染色体的提取与分析
Reem Swidah,Marco Monti,Daniela Delneri
Reem Swidah
The protocol for Extraction and Analysis of Small Yeast-Chromosomes (EASY-C) is a transformative, rapid, cost-effective, and user-friendly method designed for the efficient isolation of artificial synthetic mini-chromosomes (~42-52 kb) and ...
Development and characterization of pNarsenic: a naringenin-inducible biosensor for arsenic in Escherichia coli [0.03%]
大肠杆菌中一种可诱导表达的生物传感器pNarsenic的开发及鉴定：用于检测砷元素
Vincent Crabbe,Ezgi Unal,Stijn De Graeve et al.
Vincent Crabbe et al.
Whole-cell biosensors detecting the heavy metal arsenic have been widely studied for their potential in environmental monitoring. And while inducible biosensors have been shown to be an effective tool to tune the operational range, a thorou...
RAPID-DASH: fast and efficient assembly of guide RNA arrays for multiplexed CRISPR-Cas9 applications [0.03%]
快速高效的用于多重CRISPR-Cas9应用的导向RNA阵列装配方法 Rapids-Dash
Asfar Lathif Salaudeen,Nicholas Mateyko,Carl G de Boer
Asfar Lathif Salaudeen
Guide RNA (gRNA) arrays can enable targeting multiple genomic loci simultaneously using CRISPR-Cas9. In this study, we present a streamlined and efficient method to rapidly construct gRNA arrays with up to 10 gRNA units in a single day. We ...
Emerging trends in genome integration tools for precision engineering of diverse bacterial species [0.03%]
多种细菌物种基因组整合工具的精准工程化新兴趋势
Riesa K W Rohmat,Thea C T Irvine,Shivang Hina-Nilesh Joshi et al.
Riesa K W Rohmat et al.
The ability to precisely insert DNA payloads into a genome enables the comprehensive engineering of cellular phenotypes and the creation of new biotechnologies. To achieve such modifications, the most widely used techniques rely on a host c...
Harnessing mass spectrometry-based proteomics for continuous directed evolution [0.03%]
利用基于质谱的蛋白质组学进行连续定向进化
Katharina Belt,David Obe,Mark A Wilson et al.
Katharina Belt et al.
Continuous directed evolution is a powerful Synthetic Biology tool to engineer proteins with desired functions in vivo. Mimicking natural evolution, it involves repeated cycles of high-frequency mutagenesis, selection, and replication withi...