Christoph Crocoll,Barbara Ann Halkier,Meike Burow
Christoph Crocoll
Recent advances in liquid chromatography and mass spectrometry have made it possible to increase the throughput of phytochemical analyses at high sensitivity. These improvements have made it more feasible to monitor metabolic processes at t...
Cui-Cui Yin,Biao Ma,Wei Wang et al.
Cui-Cui Yin et al.
There has been a surge of interest in the molecular biology of RNA. We describe a simple, fast, and convenient protocol for isolating total RNA from rice (Oryza sativa) seedlings and young tissues/organs. RNA obtained using this method can ...
Virus-Induced Gene Silencing and Transient Gene Expression in Soybean (Glycine max) Using Bean Pod Mottle Virus Infectious Clones [0.03%]
利用豆荚斑驳病毒感染克隆在大豆中进行病毒诱导的基因沉默和瞬时转化
Steven A Whitham,Lori M Lincoln,R V Chowda-Reddy et al.
Steven A Whitham et al.
Virus-induced gene silencing (VIGS) is a powerful and rapid approach for determining the functions of plant genes. The basis of VIGS is that a viral genome is engineered so that it can carry fragments of plant genes, typically in the 200 to...
Quantitative Auxin Metabolite Profiling Using Stable Isotope Dilution UHPLC-MS/MS [0.03%]
采用稳定同位素稀释UHPLC-MS/MS法进行定量的生长素代谢产物谱分析
Ondřej Novák,Aleš Pěnčík,Ota Blahoušek et al.
Ondřej Novák et al.
Auxin, a plant hormone, is able to stimulate growth and to control developmental processes. To get a deeper level of understanding of how auxin activity is regulated in planta, we have developed a sensitive and selective mass spectrometry-b...
An Improved Transient Expression System Using Arabidopsis Protoplasts [0.03%]
一种利用拟南芥原生质体制备的改良瞬时表达体系
Yangrong Cao,Hong Li,An Q Pham et al.
Yangrong Cao et al.
Transient gene expression in protoplasts provides a powerful tool to study protein expression, protein localization, protein-protein association, and gene expression regulation, etc. There are several methods including electroporation, whic...
High-Throughput Yeast One-Hybrid Screens Using a Cell Surface gLUC Reporter [0.03%]
基于细胞表面gLUC报告基因的高通量酵母单杂交筛选
Zheng Li,Katia Bonaldi,S Earl Kang et al.
Zheng Li et al.
Gene-centered yeast one-hybrid (Y1H) screens using arrayed genome-wide transcription factor (TF) clone collections provide a simple and effective strategy to identify TF-promoter interactions using a DNA fragment as bait. In an effort to im...
Using Xenopus laevis Oocytes to Functionally Characterize Plant Transporters [0.03%]
利用非洲爪蟾卵母细胞研究植物转运蛋白的功能特性
Sharon Pike,Michaela S Matthes,Paula McSteen et al.
Sharon Pike et al.
Functionally characterizing plant membrane transport proteins is challenging. Typically, heterologous systems are used to study them. Immature eggs (oocytes) of the South African clawed frog Xenopus laevis are considered an ideal expression...
Zhentian Lei,Barbara W Sumner,Anil Bhatia et al.
Zhentian Lei et al.
Flavonoids are a class of specialized metabolites found in many different plant species. They protect against UV radiation, scavenge reactive oxygen species, are involved in plant defense responses, and are associated with plant-microorgani...
A High-Throughput Absolute-Level Quantification of Protein-Bound Amino Acids in Seeds [0.03%]
一种高通量的种子结合型氨基酸绝对定量方法
Abou Yobi,Ruthie Angelovici
Abou Yobi
In this unit, we describe a high-throughput absolute quantification protocol for 16 protein-bound amino acids (PBAAs) that combines a microscale protein hydrolysis step and an absolute quantification step using multiple reaction monitoring-...
Enhancing Phenotyping and Molecular Analysis of Plant Root System Using Ultrasound Aeroponic Technology [0.03%]
基于超声波气培技术的植物根系表型分析及分子模块鉴定研究进展
Lise Pingault,Prince Zogli,Jennifer Brooks et al.
Lise Pingault et al.
Several plant growth systems are available to enhance the observation of the root system (e.g., hydroponic and aeroponic plant growth systems, use of transparent soils, etc.). This article describes the use of the ultrasound aeroponic syste...