Prediction and Characterization of miRNA/Target Pairs in Non-Model Plants Using RNA-seq [0.03%]
利用RNA测序技术预测并表征非模型植物的miRNA及其靶基因对
Kira C M Neller,Alexander Klenov,Katalin A Hudak
Kira C M Neller
Plant microRNAs (miRNAs) are ∼20- to 24-nucleotide small RNAs that post-transcriptionally regulate gene expression of mRNA targets. Here, we present a workflow to characterize the miRNA transcriptome of a non-model plant, focusing on miRNA...
Agrobacterium-Mediated Transformation of Brachypodium distachyon [0.03%]
农杆菌介导的Brachypodium distachyon遗传转化体系的建立及基因功能分析研究进展
Fengjuan Chen,Qi Liu,John P Vogel et al.
Fengjuan Chen et al.
Brachypodium distachyon is an excellent model system for the grasses and has been adopted as a research organism by many laboratories around the world. It has all of the biological traits required for a model system, including small stature...
Universal Methods for Transgene Induction Using the Dexamethasone-Inducible Transcription Activation System pOp6/LhGR in Arabidopsis and Other Plant Species [0.03%]
用于模式植物及其他物种转基因表达的通用方法：以二氢睾酮和地塞米松为诱导物的新型LhGR/pOp6系统
Marketa Samalova,Charlotte Kirchhelle,Ian Moore
Marketa Samalova
Use of chemically inducible systems for transgene expression is a crucial requirement for modern plant biology research, as it allows (1) expression of transgenes that compromise plant viability or fertility when constitutively expressed an...
Matthew Zinkgraf,Kathy Haiby,Meric C Lieberman et al.
Matthew Zinkgraf et al.
Establishing efficient functional genomic systems for creating and characterizing genetic variation in forest trees is challenging. Here we describe protocols for creating novel gene-dosage variation in Populus through gamma-irradiation of ...
Measurement of Enzyme Activities and Optimization of Continuous and Discontinuous Assays [0.03%]
酶活性测定及连续和非连续测定法的优化
Camille Bénard,Yves Gibon
Camille Bénard
The measurement of enzyme activities represents an important step towards the understanding of biological networks. Continuous or discontinuous assays can be used, as well as highly sensitive assays, depending on the abundance of the enzyme...
An Efficient Targeted Mutagenesis System Using CRISPR/Cas in Monocotyledons [0.03%]
单子叶植物中使用CRISPR/Cas的高效靶向诱变系统
Zhen Liang,Yuan Zong,Caixia Gao
Zhen Liang
Precise genome modification using artificial nucleases is a powerful tool for in-depth understanding of gene functions and for creating new varieties. The CRISPR/Cas system, derived from an adaptive immunity system in bacteria and archaea, ...
Rice (Oryza sativa) Protoplast Isolation and Its Application for Transient Expression Analysis [0.03%]
水稻(Oryza sativa)原生质体分离及瞬时表达分析研究
Feng He,Songbiao Chen,Yuese Ning et al.
Feng He et al.
Rice (Oryza sativa) is not only the staple food for half of the world's population but also a model monocot plant for molecular biology studies. Although rice genes have been extensively investigated in the last two decades, the functions o...
Soybean (Glycine max) Mutant and Germplasm Resources: Current Status and Future Prospects [0.03%]
大豆(Glycine max)突变体和种质资源的现状及未来展望
Benjamin W Campbell,Robert M Stupar
Benjamin W Campbell
Genetic bottlenecks during domestication and modern breeding limited the genetic diversity of soybean (Glycine max (L.) Merr.). Therefore, expanding and diversifying soybean genetic resources is a major priority for the research community. ...
Generation of Stable Transgenic Rice (Oryza sativa L.) by Agrobacterium-Mediated Transformation [0.03%]
农杆菌介导的稳定转基因水稻(Oryza sativa L.)系的获得
Yi Zhang,Jun Li,Caixia Gao
Yi Zhang
Rice (Oryza sativa L.) is the staple food for more than half of the world's population, and has also become an important model monocot. As a result, numerous genetic transformation protocols have been developed to improve and better underst...
Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones [0.03%]
用特异染色体BAC克隆绘制拟南芥染色体图像
Terezie Mandáková,Martin A Lysak
Terezie Mandáková
Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of chromosome-specific DNA probes to identify large chromosome regions, chromosome arms, and whole chromosomes. For CP and CCP (comparative chromosome painting) in...