Bisulfite Sequence Analyses Using CyVerse Discovery Environment: From Mapping to DMRs [0.03%]
基于CyVerse发现环境的重亚硫酸盐高通量测序数据分析：从reads比对到DMR检测全流程解决方案
Jawon Song,Greg Zynda,Samuel Beck et al.
Jawon Song et al.
Epigenetic modification of DNA through methylation is known to be involved in multiple biological processes such as gene suppression. However, the exact mechanism of how DNA methylations play their part is yet unclear. In mammals, CpG islan...
Yuan Hu Xuan,Chul Min Kim,Byoung Il Je et al.
Yuan Hu Xuan et al.
Rice (Oryza sativa) is the most important consumed staple food for a large and diverse population worldwide. Since databases of genomic sequences became available, functional genomics and genetic manipulations have been widely practiced in ...
Telomere-Mediated Chromosomal Truncation for Generating Engineered Minichromosomes in Maize [0.03%]
端粒介导的染色体截短技术在玉米染色体工程中的应用及其机制研究
Nathan C Swyers,Jon P Cody,Morgan E McCaw et al.
Nathan C Swyers et al.
Minichromosomes have been generated in maize using telomere-mediated truncation. Telomere DNA, because of its repetitive nature, can be difficult to manipulate. The protocols in this unit describe two methods for generating the telomere DNA...
Transposon Mutagenesis and Analysis of Mutants in UniformMu Maize (Zea mays) [0.03%]
均匀插入Mutator转座子的玉米突变体的诱变和分析(Zea mays)
Peng Liu,Donald R McCarty,Karen E Koch
Peng Liu
A wealth of maize mutants is now available with known sites of transposon insertions in over 45% of maize genes. Materials can be obtained free of charge from the UniformMu public resource through MaizeGDB.org or directly from the Maize Gen...
Morgan McCaw,Nathan Swyers,Nathaniel Graham et al.
Morgan McCaw et al.
High-quality preparations of chromosomes are useful for many purposes. To prepare metaphase chromosome spreads in maize, root tips are harvested and treated with nitrous oxide to stop cell division at metaphase before being fixed in acetic ...
Youjun Zhang,Roberto Natale,Adilson Pereira Domingues Júnior et al.
Youjun Zhang et al.
Enzyme-enzyme interactions can be discovered by affinity purification mass spectrometry (AP-MS) under in vivo conditions. Tagged enzymes can either be transiently transformed into plant leaves or stably transformed into plant cells prior to...
Identification and Functional Investigation of Genome-Encoded, Small, Secreted Peptides in Plants [0.03%]
植物基因组编码的小分泌肽的鉴定和功能研究
Clarissa Boschiero,Peter K Lundquist,Sonali Roy et al.
Clarissa Boschiero et al.
Hundreds to thousands of small secreted peptides (SSPs) are encoded in plant genomes but have been overlooked, and most remain unannotated and unstudied. Despite their low profile, they have been found to confer dramatic effects on growth a...
Insertion Pool Sequencing for Insertional Mutant Analysis in Complex Host-Microbe Interactions [0.03%]
复杂宿主-微生物相互作用中的插入突变分析的插入池测序方法
Simon Uhse,Florian G Pflug,Arndt von Haeseler et al.
Simon Uhse et al.
Insertional mutant libraries of microorganisms can be applied in negative depletion screens to decipher gene functions. Because of underrepresentation in colonized tissue, one major bottleneck is analysis of species that colonize hosts. To ...
Profiling Cell Wall Monosaccharides and Nucleotide-Sugars from Plants [0.03%]
植物细胞壁单糖和核苷酸糖的图谱分析方法学研究进展
Carsten Rautengarten,Joshua L Heazlewood,Berit Ebert
Carsten Rautengarten
The cell wall is an intricate mesh largely composed of polysaccharides that vary in structure and abundance. Apart from cellulose biosynthesis, the assembly of matrix polysaccharides such as pectin and hemicellulose occur in the Golgi appar...
René Schneider,Arun Sampathkumar,Staffan Persson
René Schneider
The cytoskeleton is key to many essential processes in a plant cell, e.g., growth, division, and defense. Contrary to what "skeleton" implies, the cytoskeleton is highly dynamic, and is able to re-organize itself continuously. The advent of...