Isolation of Novel MAMP-like Activities and Identification of Cognate Pattern Recognition Receptors in Arabidopsis thaliana Using Next-Generation Sequencing (NGS)-Based Mapping [0.03%]
利用基于下一代测序技术的图位克隆法在拟南芥中分离新型MAMP样的活性并鉴定相应的模式识别受体
Li Fan,Eunyoung Chae,Andrea A Gust et al.
Li Fan et al.
Activation of pattern-triggered plant immunity requires recognition of microbe-derived molecular patterns (MAMPs) by plant-encoded pattern recognition receptors (PRRs). Many plant PRRs are found in selected plant genera only. Transfer of si...
Extracellular Alkalinization Assay for the Detection of Early Defense Response [0.03%]
细胞外碱化测定法检测早期防御反应
Natalia Moroz,Alisa Huffaker,Kiwamu Tanaka
Natalia Moroz
Plant recognition of invading organisms occurs through identification of foreign molecules associated with attackers and of self-derived, damage-associated molecules. Perception of these molecules activates signaling processes including dyn...
Plant Growth-Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities [0.03%]
植物促生细菌(PGPR)的分离及促生作用筛选
Adriana Ambrosini,Luciane M P Passaglia
Adriana Ambrosini
Plant roots are associated with numerous and diverse types of beneficial and pathogenic microorganisms. Plant growth-promoting (rhizo)bacteria (PGPB or PGPR) are isolated from plants crops worldwide, and many of them are used as agricultura...
Nicola J Patron
Nicola J Patron
Parallel DNA assembly methods allow multiple fragments of DNA to be compiled in a desired order in a single reaction. Several methods enable the efficient one-step assembly of multiple DNA parts into a suitable plasmid acceptor at high effi...
Generation of Transgenic Soybean (Glycine max) via Particle Bombardment of Embryogenic Cultures [0.03%]
显性荧光标记的转基因大豆（Glycine max）的培养体胚胎发生法生成
John J Finer
John J Finer
This protocol describes one method for generating transgenic soybean (Glycine max) using particle bombardment of embryogenic cultures. Embryogenic cultures consist of proliferating masses of somatic embryos and are initially obtained from t...
Live-Cell Imaging of Meiotic Spindle and Chromosome Dynamics in Maize (Zea mays) [0.03%]
玉米（Zea mays）减数分裂纺锤体和染色体的活细胞成像
Natalie J Nannas,R Kelly Dawe
Natalie J Nannas
Live-cell imaging is a powerful tool that allows investigators to directly observe the dynamics of cellular processes. Live imaging has proven particularly useful in studying mitotic and meiotic chromosome segregation, where the assembly of...
Haoyang Xin,Yue Lan,Jisen Shi et al.
Haoyang Xin et al.
A technique that produces large numbers of good quality pachytene chromosome preparations has been developed for Populus. Anthers at the pachytene stage of meiosis are used as materials. There are two main modifications in our method relati...
Manuela Peukert,Wai Li Lim,Udo Seiffert et al.
Manuela Peukert et al.
Higher plants are composed of a multitude of tissues with particular functions, reflected by distinct profiles of transcripts, proteins, and metabolites. Although the rapid development of "omics" technologies has advanced plant science trem...
Chemical Mutagenesis of Seed and Vegetatively Propagated Plants Using EMS [0.03%]
用EMS诱变种子和营养繁殖植物化学诱变种子和营养体移植植物以EMS进行化学诱变
Joanna Jankowicz-Cieslak,Bradley J Till
Joanna Jankowicz-Cieslak
Chemical mutagenesis provides an inexpensive and straightforward way to generate a high density of novel nucleotide diversity in the genomes of plants and animals. Mutagenesis therefore can be used for functional genomic studies and also fo...
Morgan McCaw,Nathaniel Graham,Jon Cody et al.
Morgan McCaw et al.
Fluorescence In Situ Hybridization (FISH) is the annealing of fluorescent DNA probes to their complementary sequences on prepared chromosomes and subsequent visualization with a fluorescent microscope. In maize, FISH is useful for distingui...