Xianwei Song,Xiaofeng Cao
Xianwei Song
Rice (Orzya sativa) small RNAs regulate almost all biological processes and agronomic traits. Characterization and dissection of small RNA functions have become an important part of rice functional genomic research. Isolation of small RNAs ...
Stephen J Anderson,Matthew R Willmann,Brian D Gregory
Stephen J Anderson
RNA secondary structure and RNA-protein interactions are necessary for maintaining biological functionality and regulatory mechanisms within eukaryotic transcriptomes. Determining the structural characteristics and protein-bound sites of RN...
Katalin Tóth,Josef Batek,Gary Stacey
Katalin Tóth
Legumes-because of their nitrogen-fixing capacity-have both ecological and agronomic importance, and are also the major plant protein source for animal consumption. The model legume species are Lotus japonicus, Medicago truncatula, and soyb...
Seth D Findley,James A Birchler,Gary Stacey
Seth D Findley
This unit presents a highly reliable protocol to produce and screen metaphase chromosome spreads from root tip cell suspensions of soybean (Glycine max), or other legumes. The procedures represent soybean-optimized versions of protocols dev...
Genotyping-by-Sequencing [0.03%]
序列分型技术
Jason G Wallace,Sharon E Mitchell
Jason G Wallace
Genotyping-by-sequencing (GBS) refers to a suite of related methods that obtain genotype data from samples by using restriction enzyme digestion followed by high-throughput sequencing. GBS is a refinement of restriction site-associated DNA ...
Sandra M Mathioni,Atul Kakrana,Blake C Meyers
Sandra M Mathioni
Plant small RNAs are ∼20 to 24 nucleotide noncoding RNAs that typically have repressive regulatory roles in gene expression, functioning at the transcriptional or post-transcriptional level. This influence on regulation of developmental an...
Whole-Plant Manual and Image-Based Phenotyping in Controlled Environments [0.03%]
人工控制环境下的全株系手册及基于图像表型研究
Erica Agnew,Adam Bray,Eric Floro et al.
Erica Agnew et al.
Phenotypic measurements and images of crops grown under controlled-environment conditions can be analyzed to compare plant growth and other phenotypes from diverse varieties. Those demonstrating the most favorable phenotypic traits can then...
Seth D Findley,James A Birchler,Gary Stacey
Seth D Findley
This article presents protocols for fluorescence in situ hybridization (FISH) in the cultivated soybean, Glycine max. The protocols represent soybean-optimized versions developed for maize. We describe the use of two different probes types:...
Liana T Burghardt,Nevin D Young,Peter Tiffin
Liana T Burghardt
Genome-wide association studies (GWAS) have developed into a valuable approach for identifying the genetic basis of phenotypic variation. In this article, we provide an overview of the design, analysis, and interpretation of GWAS. First, we...
Improved Genetic Transformation of Sugarcane (Saccharum spp.) Embryogenic Callus Mediated by Agrobacterium tumefaciens [0.03%]
农杆菌介导的甘蔗胚性 callus 的遗传转化体系的改良
Marcos Fernando Basso,Bárbara Andrade Dias Brito da Cunha,Ana Paula Ribeiro et al.
Marcos Fernando Basso et al.
Sugarcane (Saccharum spp.) is a monocotyledonous semi-perennial C4 grass of the Poaceae family. Its capacity to accumulate high content of sucrose and biomass makes it one of the most important crops for sugar and biofuel production. Conven...