Erica D LaMontagne,Carina A Collins,Scott C Peck et al.
Erica D LaMontagne et al.
Cellular membranes define the boundaries between organelles and the cytosol or the extracellular environment, thus providing functional separation between subcellular compartments. In addition, membranes assist in a diverse range of cellula...
Soluble Sugar and Starch Extraction and Quantification from Maize (Zea mays) Leaves [0.03%]
从玉米叶中提取和定量可溶性糖和淀粉
Kristen A Leach,David M Braun
Kristen A Leach
An easy-to-perform protocol for isolating and quantifying soluble sugars (sucrose, glucose, and fructose) and starch from maize (Zea mays) leaf tissue is described. The method has been optimized to extract non-structural carbohydrates (NSC)...
Kai Wang,Weichang Yu
Kai Wang
Fluorescence in situ hybridization (FISH) is widely used in cytogenetics to determine the localization of DNA sequences on target chromosomes, to provide visible information regarding the physical position of DNA sequences, to determine the...
Minoru Murata
Minoru Murata
In Arabidopsis thaliana, various attempts have been made to create artificial chromosomes as a new tool for cytological and genetic analyses. However, most of the efforts have been unsuccessful until recently. Most eukaryotic chromosomes ar...
Nathan J Bivens,Mingyi Zhou
Nathan J Bivens
Next-generation sequencing (NGS) technologies have revolutionized the study of genomics with an ever-expanding list of applications. RNA-Seq has emerged as a powerful method, applying transcriptome analysis to a wider range of organisms-mos...
Chunhui Xu,Weichang Yu
Chunhui Xu
Chromosomes are the carriers of genetic material in biological organisms. Each chromosome has three essential components: a centromere, telomeres, and origins of replication. The understanding of the essential structural and functional orga...
Purification of Translating Ribosomes and Associated mRNAs from Soybean (Glycine max) [0.03%]
从大豆（Glycine max）中纯化翻译中的核糖体及其相关mRNA
Norma A Castro-Guerrero,Yaya Cui,David G Mendoza-Cozatl
Norma A Castro-Guerrero
Cell identity and function are largely determined by specific gene expression patterns and ultimately by the proteome. Current high-throughput sequencing technologies offer the possibility of quantifying gene expression at high resolution, ...
Genomic DNA Isolation from Maize (Zea mays) Leaves Using a Simple, High-Throughput Protocol [0.03%]
采用一种简单高效的透析法分离玉米（Zea mays）叶片基因组DNA
Kristen A Leach,Paula C McSteen,David M Braun
Kristen A Leach
A simple, robust, inexpensive, high-throughput method for isolating genomic DNA from maize (Zea mays) leaf tissues is described. The DNA obtained using this extraction protocol is suitable for polymerase chain reaction (PCR) genotyping, whi...
Terezie Mandáková,Martin A Lysak
Terezie Mandáková
Despite its small nuclear genome and minute chromosomes, the model plant Arabidopsis thaliana has become a well established model in the field of plant cytogenetics. Since 2000, most cytogenetic and epigenetic approaches have been developed...
Maize (Zea mays) Hi-II Transformation via Agrobacterium-Mediated T-DNA Transfer [0.03%]
农杆菌介导的T-DNA转移法诱导玉米愈伤组织高效转化体系的研究
Hyeyoung Lee,Zhanyuan J Zhang
Hyeyoung Lee
Genetic transformation of maize via Agrobacterium tumefaciens is still more art than science, with different researchers achieving substantially different transformation results. This article describes our advanced Agrobacterium-mediated tr...