Using CRISPR/ttLbCas12a for in planta Gene Targeting in A. thaliana [0.03%]
使用CRISPR/ttLbCas12a在拟南芥中进行基因定位
Laura Merker,Patrick Schindele,Holger Puchta
Laura Merker
CRISPR/Cas systems enable gene editing through the induction of site-specific DNA double-strand breaks (DSB). However, the nature of the induced modification highly depends on the mechanism used for DNA DSB repair. Non-homologous end joinin...
Adenine Nucleotide and Nicotinamide Adenine Dinucleotide Measurements in Plants [0.03%]
植物中腺嘌呤核苷酸和烟酰胺腺嘌呤二核苷酸的测定
Youjun Zhang,Ina Krahnert,Antje Bolze et al.
Youjun Zhang et al.
As the principal co-factors of many metabolic pathways, the measurement of both adenine nucleotides and nicotinamide adenine dinucleotide provides important information about cellular energy metabolism. However, given their rapid and revers...
Design and Application of a Rotatory Device for Detecting Transient Ca2+ Signals in Response to Mechanical Stimulation Using an Aequorin-Based Ca2+ Imaging System [0.03%]
用于检测机械刺激下瞬时钙信号的基于夜光虫发光蛋白的钙成像系统旋转装置的设计与应用
Yingtong Peng,Yu Zheng,Jinrun Zhou et al.
Yingtong Peng et al.
Elevation of the cytosolic free calcium ion (Ca2+ ) concentration ([Ca2+ ]cyt ) is one of the earliest responses to biotic and abiotic stress in plant cells. Among the various Ca2+ detection systems available, aequorin-based luminescence Ca...
Robert Patalano,Jana Zech,Patrick Roberts
Robert Patalano
Plant wax lipid molecules, chiefly normal (n-) alkanes and n-alkanoic acids, are frequently used as proxies for understanding paleoenvironmental and paleoclimatic change. These are regularly analyzed from marine and lake sediments and even ...
In-Plate Quantitative Characterization of Arabidopsis thaliana Susceptibility to the Fungal Vascular Pathogen Fusarium oxysporum [0.03%]
拟南芥对血管真菌病原物尖镰刀菌的感病性测定及定量分析
Apolonio Ignacio Huerta,Christopher Kesten,Alexandra Lauren Menna et al.
Apolonio Ignacio Huerta et al.
Root vascular pathogens are some of the world's most devastating plant pathogens. However, the methods used to determine plant susceptibility to this class of pathogen are laborious, variable, and in most cases qualitative. Here we present ...
Erika Toda,Takashi Okamoto
Erika Toda
Genome-editing technology involving the targeted mutagenesis of plants using programmable nucleases has been developing rapidly and has enormous potential in next-generation plant breeding. Its application has been hindered in many cases, h...
Si Nian Char,Hyeyoung Lee,Bing Yang
Si Nian Char
Sorghum (Sorghum bicolor) fulfills the demand for bioenergy resources and also provides substantial diet calories to the world's population. Therefore, many biological studies use sorghum as a research model for improvement of the domestica...
Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis for Lipophilic Compound Profiling in Plants [0.03%]
基于液相色谱-质谱法（LC-MS）的植物疏水性化合物轮廓分析
Taly Lapidot-Cohen,Leah Rosental,Yariv Brotman
Taly Lapidot-Cohen
Lipids are fascinating due to their chemical diversity, which is especially vast in the plant kingdom thanks to the high plasticity of the plant biosynthetic machinery. Lipidomic studies aim to simultaneously analyze a large number of lipid...
Analysis of Tomato Post-Harvest Properties: Fruit Color, Shelf Life, and Fungal Susceptibility [0.03%]
番茄采后性质分析：果实颜色、货架寿命和抗真菌性
Vera Thole,Philippe Vain,Ray-Yu Yang et al.
Vera Thole et al.
A wide variety of fresh market and processing tomatoes (Solanum lycopersicum) is grown and consumed worldwide. Post-harvest losses are a major contributing factor to losses in crop productivity and can account for up to 50% of the harvest. ...
Atara Gal,Elisha Hendel,Zvi Peleg et al.
Atara Gal et al.
Root-system hydraulic conductivity (RSHC) is an important physiological characteristic that describes the inherent ability of roots to conduct water across a water-potential gradient between the root and the stem xylem. RSHC is commonly use...