Fixation and Laser Capture Microdissection of Plant Tissue for RNA Extraction and RNASeq Library Preparation [0.03%]
植物组织的固定和激光捕获微解剖以用于RNA提取和RNA-Seq文库构建
Suchitra Chavan,Elise Schnabel,Christopher Saski et al.
Suchitra Chavan et al.
In order to study the transcriptome of individual plant cells at specific points in time, we developed protocols for fixation, embedding, and sectioning of plant tissue followed by laser capture microdissection (LCM) and processing for RNA ...
Melina Altmann,Stefan Altmann,Claudia Falter et al.
Melina Altmann et al.
In this article, we describe a Y2H interaction mapping protocol for systematically screening medium-to-large collections of open reading frames (ORFs) against each other. The protocol is well suited for analysis of focused networks, for mod...
High Enrichment [13 C]-Labeling of Plants Grown Hydroponically from Seed to Seed in a Controlled 13 C-Carbon Dioxide Atmosphere Enclosure [0.03%]
以种子到种子的水培法培养植物并用控制的高浓度同位素二氧化碳环境进行13C标记
Calvin P Peters,Erin M Evans,Jerry D Cohen et al.
Calvin P Peters et al.
In vivo isotopic labeling empowers proteomic and metabolomic analyses to resolve relationships between the molecular composition, environment, and phenotype of an organism. Carbon-13 is particularly useful for plant labeling as it can be in...
Masatsugu Hashiguchi,Hidenori Tanaka,Melody Muguerza et al.
Masatsugu Hashiguchi et al.
A quarter of a century has passed since Lotus japonicus was proposed as a model legume because of its suitability for molecular genetic studies. Since then, a comprehensive set of genetic resources and tools has been developed, including re...
Using Flow Cytometry to Isolate Maize Meiocytes for Next Generation Sequencing: A Time and Labor Efficient Method [0.03%]
一种高效地从玉米中分离小孢子用于下一代测序的流式细胞术方法研究
Pearl Chang,Yu-Fang Tseng,Pao-Yang Chen et al.
Pearl Chang et al.
Meiosis is essential during sexual reproduction to generate haploid gametes. Genomic or epigenomic studies of meiosis in multicellular organisms using next-generation sequencing (NGS) methods have been limited because of the difficulty of c...
Sarah Refi Hind,Jason S Hoki,Joshua A Baccile et al.
Sarah Refi Hind et al.
The field of plant receptor biology has rapidly expanded in recent years, however the demonstration of direct interaction between receptor-ligand pairs remains a challenge. Click chemistry has revolutionized small molecule research but lack...
Profiling of transcription factor binding events by chromatin immunoprecipitation sequencing (ChIP-seq) [0.03%]
染色质免疫沉淀测序(ChIP-seq)技术分析转录因子的结合事件
Liang Song,Yusuke Koga,Joseph R Ecker
Liang Song
In multi-cellular organisms, gene expression is orchestrated by thousands of transcription factors (TF). Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a robust tool to investigate gene expression because this technique ...
Zuodong Jiang,Chase Kempinski,Joe Chappell
Zuodong Jiang
Terpenes/terpenoids constitute one of the largest classes of natural products, this is due to the incredible chemical diversity that can arise from the biochemical transformations of the relatively simple prenyl diphosphate starter units. A...