Transformation of Recalcitrant Sorghum Varieties Facilitated by Baby Boom and Wuschel2 [0.03%]
Baby Boom和Wuschel2介导的顽拗型高粱品种转化研究得进展
Kimberly Nelson-Vasilchik,Joel Hague,Muruganantham Mookkan et al.
Kimberly Nelson-Vasilchik et al.
Most reliable transformation protocols for cereal crops, including sorghum (Sorghum bicolor L. Moench), rely on the use of immature embryo explants to generate embryogenic callus cells that are then transformed using Agrobacterium- or parti...
Muruganantham Mookkan,Kimberly Nelson-Vasilchik,Joel Hague et al.
Muruganantham Mookkan et al.
Maize B73 is a reference genome and has long been a major resource for genetics and molecular biology research. We have developed an efficient B73 transformation protocol by enabling somatic embryogenesis through differential co-expression ...
Rapid and Efficient Genetic Transformation of Sorghum via Agrobacterium-Mediated Method [0.03%]
一种高效的农杆菌介导的高粱遗传转化方法
Phat Tien Do,Hyeyoung Lee,Kimberly Nelson-Vasilchik et al.
Phat Tien Do et al.
Genetic transformation via Agrobacterium-mediated methodology has been used in many sorghum studies. However, the transformation efficiency still varies significantly due to high dependence on sorghum genotypes and technical expertise. In t...
Protocol for Chromatin Immunoprecipitation of Meiotic-Stage-Specific Tomato Anthers [0.03%]
番茄花药特异性减数分裂时期的染色质免疫沉淀操作规范
Jihed Chouaref,Esther de Boer,Paul Fransz et al.
Jihed Chouaref et al.
Interactions occurring between DNA and proteins across the nuclear genome regulate numerous processes, including meiosis. Meiosis ensures genetic variation and balanced segregation of homologous chromosomes. It involves complex DNA-protein ...
Labeling Maize (Zea mays L.) Leaves with 15 NH4+ and Monitoring Nitrogen Incorporation into Amino Acids by GC/MS Analysis [0.03%]
用15NH4+标记玉米叶片并用气质联用仪测定氮素向氨基酸中的转移
Caroline Cukier,Peter J Lea,Rafael Cañas et al.
Caroline Cukier et al.
The human body contains approximately 3.2% nitrogen (N), mainly present as protein and amino acids. Although N exists at a high concentration (78%) in the air, it is not readily available to animals and most plants. Plants are however able ...
Carlos Ortiz-Ramírez,Edgar Demesa Arevalo,Xiaosa Xu et al.
Carlos Ortiz-Ramírez et al.
Maize is one the most widely cultivated crops worldwide and an important model system for the study of genetics and cytogenetics. Although the availability of a genome sequence has enabled new quantitative genomic studies, developing method...
Profiling Interactome Networks with the HaloTag-NAPPA In Situ Protein Array [0.03%]
带有HaloTag的NAPPA原位蛋白芯片绘制互作组网络
Junshi Yazaki,Mary Galli,Alice Y Kim et al.
Junshi Yazaki et al.
Physical interactions between proteins and other molecules can be evaluated at a proteome scale using protein arrays, a type of high-throughput pulldown assay. We developed a modified in situ protein array known as the nucleic acid programm...
Nagib Ahsan,Rashaun S Wilson,Jay J Thelen
Nagib Ahsan
Among targeted proteomic techniques, AQUA-MRM is considered as one of the most reliable for accurate protein quantitation. This method displays high sensitivity, specificity, and reproducibility compared to many common biochemical technique...
Highly Efficient Agrobacterium-Mediated Transformation of Potato (Solanum tuberosum) and Production of Transgenic Microtubers [0.03%]
高效地介导马铃薯（Solanum tuberosum）的农杆菌转化及转基因微薯的产生
Melanie Craze,Ruth Bates,Sarah Bowden et al.
Melanie Craze et al.
The following method enables the rapid production of transgenic potato plants and microtubers for gene validation and expression, or promoter studies. The method is highly efficient, with reproducible transformation efficiencies of at least...
Luciferase Complementation Assay for Protein-Protein Interactions in Plants [0.03%]
植物中用于蛋白质-蛋白质相互作用的荧光素酶互补检测方法
Zhaoyang Zhou,Guozhi Bi,Jian-Min Zhou
Zhaoyang Zhou
Constitutive and dynamic protein-protein interactions are fundamental to all aspects of cellular processes. Compared to other techniques measuring protein-protein interactions in plants, the luciferase complementation assay has a number of ...