An Inexpensive, Easy-to-Use, and Highly Customizable Growth Chamber Optimized for Growing Large Plants [0.03%]
一种价格低廉、易于使用且高度可定制的生长室，适合种植大型植物
Thu M Tran,David M Braun
Thu M Tran
The ability to grow plants in highly controlled and reproducible environments is a critical factor for successful plant biology experiments. This protocol describes a simple and inexpensive method for constructing a fully automatic controll...
Drug Affinity Responsive Target Stability (DARTS) to Resolve Protein-Small Molecule Interaction in Arabidopsis [0.03%]
基于药物亲和性稳定蛋白质靶标的植物蛋白药物相互作用研究技术(DARTS)
Cecilia Rodriguez-Furlan,Chunhua Zhang,Natasha Raikhel et al.
Cecilia Rodriguez-Furlan et al.
Target identification remains a challenging step in plant chemical genomics approaches. Drug affinity responsive target stability (DARTS) represents a straightforward technique to identify small molecules' protein targets and assist in the ...
X-Ray Computed Tomography of Crop Plant Root Systems Grown in Soil [0.03%]
生长在土壤中的农作物根系的x射线计算机断层扫描技术(X-CT)
Stefan Mairhofer,Tony Pridmore,James Johnson et al.
Stefan Mairhofer et al.
Non-destructive methods to quantify the root system architecture of a plant grown in soil are essential to aid our understanding of the factors that impact plant root development in natural environments. With environmental change threatenin...
Agrobacterium-Mediated Transformation of Oilseed Rape (Brassica napus) [0.03%]
芸苔属作物（甘蓝型油菜）的农杆菌介导转化体系的研究进展及应用展望
Ruth Bates,Melanie Craze,Emma J Wallington
Ruth Bates
Oilseed rape (Brassica napus) is a commercially important member of the Brassicacea family. It is grown for its edible and industrial oils as well as for animal feed. Genetic transformation technology has been used to study gene function an...
Marie Garmier,Laurent Gentzbittel,Jiangqi Wen et al.
Marie Garmier et al.
Medicago truncatula was chosen by the legume community, along with Lotus japonicus, as a model plant to study legume biology. Since then, numerous resources and tools have been developed for M. truncatula. These include, for example, its ge...
BiBAC Modification and Stable Transfer into Maize (Zea mays) Hi-II Immature Embryos via Agrobacterium-Mediated Transformation [0.03%]
用于玉米稳定转化的BiBAC载体构建及感受态幼胚的侵染试验
Jon P Cody,Nathaniel D Graham,James A Birchler
Jon P Cody
Binary Bacterial Artificial Chromosomes (BiBAC) are large insert cloning vectors that contain the necessary features required for Agrobacterium-mediated transformation. However, the large size of BiBACs and low-copy number in Escherichia co...
Isolation, Library Preparation, and Bioinformatic Analysis of Historical and Ancient Plant DNA [0.03%]
历史和古代植物DNA的分离、文库制备及生物信息学分析
Sergio M Latorre,Patricia L M Lang,Hernán A Burbano et al.
Sergio M Latorre et al.
The ability to sequence DNA retrieved from ancient and historical material plays a crucial role in reinforcing evolutionary and anthropological inference. While the focus of the field is largely on analyzing DNA from ancient hominids and ot...
Sandra Thibivilliers,Dirk Anderson,Marc Libault
Sandra Thibivilliers
The characterization of the transcriptional similarities and differences existing between plant cells and cell types is important to better understand the biology of each cell composing the plant, to reveal new molecular mechanisms controll...
Selective Enrichment Coupled with Proteomics to Identify S-Acylated Plasma Membrane Proteins in Arabidopsis [0.03%]
选择性富集结合蛋白组学识别阿拉伯芥中的S-酰化质膜蛋白
Lijuan Zhou,Mowei Zhou,Marina A Gritsenko et al.
Lijuan Zhou et al.
Protein S-acylation, predominately in the form of palmitoylation, is a reversible lipid post-translational modification on cysteines that plays important roles in protein localization, trafficking, activity, and complex assembly. The functi...
Monika Kosmacz,Aleksandra Skirycz
Monika Kosmacz
Stress granules (SGs) are ubiquitous nonmembrane-bound assemblies of protein and mRNA formed under stress conditions associated with stalled translation. SGs are evolutionarily conserved across eukaryotes. The canonical function of SGs is t...