Reverse Transcriptase Activity with CRISPR (REACTR) Assay for Rapid and User-Friendly Therapeutic Drug Monitoring in Cytomegalovirus Care [0.03%]
用于巨细胞病毒治疗的快速简便的治疗药物监测的CRISPR反式录酶活性测定法(REACTR)
Willow A Chernoske,Maya A Singh,Carrie H Lin et al.
Willow A Chernoske et al.
Cytomegalovirus (CMV) can cause severe disease and death in infants and immunocompromised people. The first-line drug ganciclovir has high pharmacokinetic variability which leads to significant rates of underexposure or toxicity. Intracellu...
Biochemical and Structural Characterization of Two-domain Glycoside Hydrolase PgaB from Serratia marcescens and Its Application for S. aureus Biofilm Degradation [0.03%]
产生滑动色素杆菌两结构域糖苷水解酶PgaB的生化和结构表征及其降解金黄色葡萄球菌生物被膜的应用研究
Amanda Freitas Cruz,Pedro Ricardo Vieira Hamann,Francisco Eduardo Gontijo Guimaraes et al.
Amanda Freitas Cruz et al.
Antimicrobial resistance (AMR) is a critical global health threat, with projections estimating up to 10 million deaths annually by 2050. One of the strategies for developing bacterial AMR is the formation of microbial biofilms (BFs). Thus, ...
Inhibiting Bacterioferritin Iron Release Induces Iron Starvation and Metabolic Downshift, Potentiating Aminoglycosides in P. aeruginosa Biofilms [0.03%]
抑制泛菌铁蛋白释铁可诱导潘氏铁饥饿和代谢下降，增强其对铜绿假单胞菌生物被膜氨基糖苷类抗生素的敏感性
Leo Fontenot,Huili Yao,Alexanndra M Behm et al.
Leo Fontenot et al.
In Pseudomonas aeruginosa, bacterioferritin contributes to regulating cytosolic iron by oxidizing Fe2+ and storing Fe3+ within its internal cavity, and binding to its cognate ferredoxin to reduce stored Fe3+ and release Fe2+ to the cytosol....
A Nanoconfined, Hydrogel-Partitioned LAMP Platform for Rapid, Extraction-Free Quantification of Mycoplasma pneumoniae [0.03%]
一种用于快速、无需提取定量肺炎支原体的纳米限制型水凝胶隔室LAMP平台
Xiaoyang Jin,Yuxuan Li,Yajun Guo et al.
Xiaoyang Jin et al.
Accurate quantification of Mycoplasma pneumoniae (M. pneumoniae) is important because pathogen load is associated with disease severity and treatment outcomes. However, quantitative polymerase chain reaction (qPCR) requires DNA extraction a...
Aerocavin Is an Antibiotic with Potent and Specific Anti-Neisserial Activity [0.03%]
新型抗生素Aerocavin具有高效的抗淋球菌活性
Gleb Pishchany,Kyra E Fryling,Vineetha Vasukuttan et al.
Gleb Pishchany et al.
Gonorrhea, caused by Neisseria gonorrhoeae, is a widespread sexually transmitted disease that is becoming resistant to all currently used antibiotics. Therefore, new therapeutics for gonorrhea are desperately needed. Here, we show that a na...
Multiple Protein-Protein Interactions Drive the Assembly and Budding of the Chikungunya Virion [0.03%]
多种蛋白质-蛋白质相互作用驱动登革病毒颗粒的组装和芽生现象
Miguel Angel Coronado-Ipiña,Shuyang Zhang,Siyu Li et al.
Miguel Angel Coronado-Ipiña et al.
The assembly of enveloped viruses is a highly orchestrated process that depends on the coupling of multiple protein-protein interactions within a membrane environment. To gain mechanistic insight into this process, we use Chikungunya virus ...
Machine Learning Approaches Using High-Throughput Profiling Data for Antibiotic Discovery [0.03%]
基于高通量表型数据的机器学习方法在抗生素发现中的应用
Inseo Kim,Junggyeong Jang,Taegwan Yun et al.
Inseo Kim et al.
Antibiotic-resistant bacterial infections continue to increase globally, creating an urgent need for new antibacterials with novel mechanisms of action. Early stages of antibiotic discovery are often limited by the difficulty of identifying...
Synthesis of 4-Aminoindole-Containing Cephalosporin Prodrugs and Their Biological Activity against Mycobacterium tuberculosis [0.03%]
含4-氨基吲哚头孢菌素前药的合成及其抗结核活性研究
Sangmi Oh,Helena I M Boshoff,Jose Santinni O Roma et al.
Sangmi Oh et al.
Indole 4-carboxamides are promising prodrugs that liberate 4-aminoindole, which poisons tryptophan biosynthesis in Mycobacterium tuberculosis (Mtb). Limiting enthusiasm for these compounds is the high rate of emergence of resistance since t...
A High-Sensitivity Colorimetric Dot-Blot Aptamer Assay Platform for SARS-CoV-2 Nucleocapsid Protein Detection in Resource-Limited Settings [0.03%]
一种灵敏的比色点杂交适配子分析平台用于在资源有限条件下检测SARS-CoV-2核衣壳蛋白
Kauser Banu,Bhairab Mondal,S Madhan Kumar et al.
Kauser Banu et al.
We developed a colorimetric dot-blot aptamer assay to address the urgent need for accessible, high-performance SARS-CoV-2 diagnostics in resource-limited settings. Our in-house 96-well dot-blot platform featuring optimized PVDF membrane flu...
Decoding the Microbiota in Health and Disease: Molecular Mechanisms and Technological Frontiers [0.03%]
健康与疾病状态下的微生物组解析：分子机制和科技前沿
Yuan Qiao,Matthew Griffin,Stavroula Hatzios
Yuan Qiao