Unique DNA impacts on transient Expi293TMPRO expression for enhancing biotherapeutic protein production [0.03%]
独特的DNA影响瞬时Expi293TM PRO表达以增强生物治疗蛋白的生产
Bo Hee Shin,Jing Zhou,Audrey M Vogt et al.
Bo Hee Shin et al.
Introduction: Mammalian transient gene expression technology is an essential tool for biotherapeutics engineering and production. Various reported transient expression systems in human embryonic kidney (HEK) 293 or Chines...
A fluffy nanofiber scaffold-based microenvironment enables a simplified, biology-centric scale-up strategy for mesenchymal stem/stromal cell culture [0.03%]
基于纳米纤维支架的微环境能够简化间充质干细胞培养的扩增流程
Masashi Iwakami,Gabrielle Erwin Gemeniano Awitan,Riku Yamamoto et al.
Masashi Iwakami et al.
Scalable suspension culture technologies are essential for the large-scale manufacturing of mesenchymal stem/stromal cells (MSCs). However, conventional microcarrier-based systems often fail to achieve scalable efficiency because cell-micro...
Viscoelastic properties of sodium hyaluronate and their mathematical optimization in intra-articular injections: a predictive model for enhancing clinical efficacy [0.03%]
玻璃酸钠的粘弹性及其在关节腔注射中的数学优化：一种增强临床疗效的预测模型
Horacio Rivarola,Stefano Guerrasio,Luciano Trinchese et al.
Horacio Rivarola et al.
Objective: To develop and validate a biomechanical and mathematical model capable of predicting the clinical efficacy of intra-articular sodium hyaluronate injections (HA) in osteoarthritis (OA), by aligning viscoelastic ...
Synergistic potential of antibiotics against an in vitro multispecies biofilm model for peri-implantitis [0.03%]
抗生素对种植体周围炎多物种生物膜的联合潜力模型的体外研究
Neele Brümmer,Karolin Behrens,Katharina Doll-Nikutta et al.
Neele Brümmer et al.
Background: Despite their widespread success, dental implants remain vulnerable to biofilm-associated infections such as peri-implantitis. Local antibiotic (AB) application may enhance treatment outcomes; however, its use...
Rethinking viral vector quantification: a microfluidic approach to standardised functional titre assays [0.03%]
反思病毒载体定量问题：用于标准化功能滴度测定的微流控方法
Daria Andreea Farcas,Charles Moore-Kelly,Philippa Stevenson et al.
Daria Andreea Farcas et al.
The quantification of lentiviral vector (LVV) potency via titration is a critical quality control step for cell and gene therapies. However, standard functional titre assays are fundamentally limited by an inability to detect multiple integ...
Integrate single-cell and transcriptome analyses to explore the prognostic genes related to TRPM4 in bladder cancer [0.03%]
整合单细胞和转录组分析探索TRPM4相关的膀胱癌预后基因
Qi Zhao,Zitong Qin,Runzhang Liu et al.
Qi Zhao et al.
Bladder cancer (BLCA) is a common malignancy of the urinary system, yet the therapeutic relevance of transient receptor potential cation channel subfamily M member 4 (TRPM4) remains unclear. By integrating single-cell and whole-genome trans...
Preoperative of balloon pulmonary angioplasty in chronic thromboembolic pulmonary hypertension using virtual balloon expansion: a pilot study [0.03%]
应用虚拟球囊扩张评估慢性血栓性肺动脉高压经皮肺动脉成形术前病变血管的可行性：一项前瞻性研究
Qianwen Liu,Yonglei Zhao,Zeping Jiang et al.
Qianwen Liu et al.
Objectives: Chronic thromboembolic pulmonary hypertension (CTEPH) demands precise balloon pulmonary angioplasty (BPA) planning to address unpredictable hemodynamic outcomes. This pilot study explores the potential of virt...
Membrane lipid order predicts potency decline in therapeutic extracellular vesicles following handling, storage, and reconstitution stress [0.03%]
膜脂有序性预测了处理、储存和重构应激后治疗性细胞外囊泡效力的下降
Kenichi Tamura,Shinji Takeoka
Kenichi Tamura
Introduction: Potency assays for therapeutic extracellular vesicles (EVs) are widely recommended, yet common quality-control readouts (e.g., protein concentration and EV marker levels) are not always stability-indicating....
Artificial intelligence and precision nursing in the operating room: transforming perioperative safety and surgical outcomes [0.03%]
人工智能与手术室精准护理：转变围术期安全和手术效果
Li Gong,Xia Zhao,Huana Zong
Li Gong
Background: The operating room represents a high-risk clinical environment where perioperative nurses play a critical role in patient safety, workflow coordination, and surgical outcomes. Recent advances in artificial int...
Modelling inflammatory endothelial dysfunction: a human in vitro platform for translational research [0.03%]
模拟炎症性内皮细胞功能障碍：一种用于转化研究的体外平台
Maria Cheremkhina,Aaron Babendreyer,Christopher T Neullens et al.
Maria Cheremkhina et al.
Introduction: Systemic inflammation presents a significant challenge to the long-term function of biohybrid implants. While endothelialisation of biohybrid implants has been shown to improve device hemocompatibility, its ...